%0 Journal Article
%T Gene expression studies of developing bovine longissimus muscle from two different beef cattle breeds
%A Sigrid A Lehnert
%A Antonio Reverter
%A Keren A Byrne
%A Yonghong Wang
%A Greg S Nattrass
%A Nicholas J Hudson
%A Paul L Greenwood
%J BMC Developmental Biology
%D 2007
%I BioMed Central
%R 10.1186/1471-213x-7-95
%X We obtained expression profiles of three individuals for each time point and genotype to allow comparisons across time and between sire breeds. Quantitative reverse transcription-PCR analysis of RNA from developing longissimus muscle was able to validate the differential expression patterns observed for a selection of differentially expressed genes, with one exception. We detected large-scale changes in temporal gene expression between the four developmental stages in genes coding for extracellular matrix and for muscle fiber structural and metabolic proteins. FSTL1 and IGFBP5 were two genes implicated in growth and differentiation that showed developmentally regulated expression levels in fetal muscle. An abundantly expressed gene with no functional annotation was found to be developmentally regulated in the same manner as muscle structural proteins. We also observed differences in gene expression profiles between the two different sire breeds. Wagyu-sired calves showed higher expression of fatty acid binding protein 5 (FABP5) RNA at birth. The developing longissimus muscle of fetuses carrying the Piedmontese mutation shows an emphasis on glycolytic muscle biochemistry and a large-scale up-regulation of the translational machinery at birth. We also document evidence for timing differences in differentiation events between the two breeds.Taken together, these findings provide a detailed description of molecular events accompanying skeletal muscle differentiation in the bovine, as well as gene expression differences that may underpin the phenotype differences between the two breeds. In addition, this study has highlighted a non-coding RNA, which is abundantly expressed and developmentally regulated in bovine fetal muscle.Genetic background and development prior to birth are known to influence the composition of bovine muscle tissue [1,2]. Prenatal muscle development is therefore a promising source of gene discovery for the molecular events that determine adult muscle
%U http://www.biomedcentral.com/1471-213X/7/95