%0 Journal Article
%T Response surface optimisation for acetone-butanol-ethanol production from cassava starch by co-culture of Clostridium butylicum and Bacillus subtilis
%A Benjamas Cheirsilp
%J Maejo International Journal of Science and Technology
%D 2011
%I Maejo University
%X Acetone-butanol-ethanol (ABE) production from cassava starch was enhanced by a syntrophic co-culture of Clostridium butylicum TISTR 1032 and high amylase producing Bacillus subtilis WD 161 without anaerobic pretreatment. The production of amylase and ABE using this co-culture were respectively 16 and 6 times higher than those using the pure culture of C. butylicum TISTR 1032. The effect of the medium components on the performance of the co-culture was investigated using response surface methodology (RSM). Among the investigated components, cassava starch and ammonium nitrate contributed a significant effect on the production of amylase and ABE, while yeast extract had less effect. Based on the optimum strategy using RSM, the ABE production by the co-culture was improved 2.2-fold compared with that obtained from the initial condition and with a minimum requirement of nitrogen source.
%K acetone¨Cbutanol¨Cethanol fermentation
%K Bacillus subtilis
%K cassava starch
%K Clostridium butylicum
%K co-culture
%U http://www.mijst.mju.ac.th/vol5/374-389.pdf