%0 Journal Article
%T Effective separation and simultaneous analysis of anabolic androgenic steroids (AAS) in their pharmaceutical formulations by a validated TLC-densitometry method
%A Syed Musharraf
%A Umair Gulzar
%J Chemistry Central Journal
%D 2012
%I BioMed Central
%R 10.1186/1752-153x-6-54
%X Separation was carried out on Al based TLC plates, pre-coated with silica gel 60F-254 using hexane and ethyl acetate (8.5:1.5, v/v). Spots at Rf 0.31£¿¡À£¿0.01, 0.34£¿¡À£¿0.01, 0.40£¿¡À£¿0.01 and 0.45£¿¡À£¿0.02 were recognized as TPP, TP, TI and TD, respectively. Quantitative analysis was done by densitometric measurements at ¦Ëmax 251 nm for all derivatives. The developed method was validated as per ICH guidelines. Method was found linear over the concentration range of 200¨C1200 ng/spot with the correlation coefficient of 0.995, 0.993, 0.995 and 0.996 for TP, TPP, TI, TD, respectively. Limit of detection for all derivatives were in the range of 16.7-22.3 ng/spot while limit of quantitation were found to be in the range of 55.7-70.9 ng/spot.The developed TLC method can be applied for the simultaneous routine analysis of testosterone derivatives in their individual and combined pharmaceutical formulations.Anabolic androgenic steroids (AAS) are mainly a group of natural or synthetic compounds that are chemically similar to the actions of androgynous testosterone, which is primarily a natural male hormone responsible for androgenic and anabolic effects observed during male adolescence and adulthood, and are modified to improve the anabolic effect of testosterone rather than its androgenic effect [1]. In addition to their medical uses, AAS have been widely misused by variety of athletes with the hope of improving their performance [2,3]. The use of AAS in sports has been banned since the mid-1970s but they are still the most misused class of drugs in sports. Moreover, steroid abuses have also become more and more prevalent outside sports.Because of the short half-life of only one hour, exogenous intake of pure testosterone do not have any effect, since only 2% of oral intake reaches to the muscles. To slow down the metabolism and receive better effect, the testosterone molecule has been modified at its 17-position which creates stronger anabolic effect and weaker androgen effect [4]
%K Testosterone derivatives
%K TLC-densitometry
%K Testosterone propionate
%K Testosterone phenyl propionate
%K Testosterone isocaproate
%K Testosterone deaconate
%U http://journal.chemistrycentral.com/content/6/1/54