%0 Journal Article
%T OBSERVATIONS REGARDING OOCYTES STORAGE POST MENDING FROM SLAUGHTER FEMALES
%A CARABA V.
%A IVAN ALEXANDRA
%A TELEA ADA
%A PACALA N.
%J Lucrari Stiintifice : Zootehnie si Biotehnologii
%D 2008
%I Facultatea de Zootehnie si Biotehnologii, Timisoara
%X The oocytes viability must be taken as an important selection parameter for successful in vitrocultivation. The ovaries were collected from the slaughterhouse and maintained at 4ˇăC for 7days. Fallowing cumulus -oocytes complexes recovery the viability was tested by two stainingmethods. For the first experiment we used 27 cumulus - oocytes complexes, stained withNeutral red and for the second experiment we used 11 cumulus - oocytes complexes stainedwith Trypan blue. Fallowing staining with Neutral red 23 cumulus - oocytes complexes wereassessed as viable (were stained in red ¨C enzymatic activity within the cells) and for the Trypanblue staining 11 cumulus - oocytes complexes were assessed as viable (remained unstained ¨Cintegers cellular membranes).
%K pig
%K oocytes
%K viability
%K IVM
%U http://usab-tm.ro/fileadmin/fzb/PDF%202008/Volumul%201/Biotechnologies/Caraba-1.pdf