%0 Journal Article
%T TWIST1, A novel androgen-regulated gene, is a target for NKX3-1 in prostate cancer cells
%A Turid Eide
%A H£¿kon Ramberg
%A Carlotta Glackin
%A Donald Tindall
%A Kristin Austlid Task¨¦n
%J Cancer Cell International
%D 2013
%I BioMed Central
%R 10.1186/1475-2867-13-4
%X Prostatic cell lines were analyzed using real time RT-PCR, chromatin immunoprecipitations (ChIP) and transfection of siRNA's and reporter constructs.We report in this paper that TWIST1 is an androgen-regulated gene under tight regulation of NKX3-1. Androgens repress the expression of TWIST1 via NKX3-1, which is a prostate--specific tumor suppressor that is down-regulated in the majority of metastatic prostate tumors. We show that NKX3-1 binds to the TWIST1 promoter and that NKX3-1 over-expression reduces the activity of a TWIST1 promoter reporter construct, whereas NKX3-1 siRNA up-regulates endogenous TWIST1 mRNA in prostate cancer cells.Our finding that NKX3-1 represses TWIST1 expression emphasizes the functional importance of NKX3-1 in regulating TWIST1 expression during prostate cancer progression to metastatic disease.
%U http://www.cancerci.com/content/13/1/4/abstract