%0 Journal Article
%T Kappa Casein Gene Polymorphism in Holstein Chinese Cattle
%A A. E. Hamza1
%A *
%A X. L. Wang and Z. P.Yang
%J Pakistan Veterinary Journal
%D 2010
%I University of Agriculture, Faisalabad
%X Kappa casein gene polymorphism has received a considerable attention because of its correlation with milk quality, composition and technological properties. The polymorphism of kappa casein gene (K-CN) was detected in Holstein Chinese cattle. A 218 bp sequence in exon IV of 319 Holstein Chinese cattle blood samples were amplified using polymerase chain reaction-single strand conformation (PCR-SSCP) technique. Sequence analysis revealed one single nucleotide polymorphism (SNP) T/C SNP in exon 1V at nucleotides (80), moreover; three genotypes TT, TC and CC were also identified with following frequencies: 0.40, 0.34 and 0.26%, respectively. The allele frequency for T and C found to be 0.6 and 0.4 %, respectively. Allele frequencies in the population fitted with Hardy¨CWeinberg equilibrium (P>0.05). Analysis of genetic polymorphism of k-casein at exon 1V exhibited medium polymorphism information content (PIC=0.36).
%K Holstein Chinese cattle
%K Kappa casein gene
%K Polymorphism
%K PCR-SSCP
%K Single nucleotide
%U http://www.pvj.com.pk/pdf-files/30_4/203-206.pdf