%0 Journal Article
%T Quantitative estimation of silybin in Silybum marianum mother tincture using high performance liquid chromatography
%A Hamrapurkar Purnima
%A Thakurdesai Leena
%A Phale Mitesh
%J International Journal of Green Pharmacy
%D 2010
%I Medknow Publications
%X Silybin is a characterising compound present in the plant Silybum marianum, used as an hepatoprotective. A simple, accurate and sensitive HPLC method was developed for estimation of silybin. The chromatographic separation was carried out on RP C18 (Waters) column (250 ¡Á 4.6 mm, 10 ¦Ìm) using mobile phase, phosphate buffer: water (65:35 % v/v) pumped at a flow rate of 1 ml/min. The eluent was monitored at 288 nm. The method is specific and linear over the range of 10-200 ng/ml. The method was statistically validated for precision, accuracy, robustness and recovery. The HPLC method can be applied for identification and on of silybin in herbal extracts of Silybum marianum.
%K HPLC
%K mother tincture
%K quantitative estimation
%K silybin
%K Silybum marianum
%U http://www.greenpharmacy.info/article.asp?issn=0973-8258;year=2010;volume=4;issue=4;spage=238;epage=241;aulast=Hamrapurkar