%0 Journal Article
%T VARIATION IN THE YAK CALPASTATIN GENE (CAST)
%A Yang G.
%A Zhou H.
%A Hu J.
%A Luo Y. and Hickford J.G.H.
%J International Journal of Genetics
%D 2012
%I Bioinfo Publications
%X Calpastatin (CAST) is a specific inhibitor of calpain (calcium-dependent cysteine protease). This study investigated the potential for variation in yak (Bos grennies) CAST. PCR-SSCP analysis of exon 6 of yak CAST revealed three unique patterns (named A-C). Sequencing of the amplicons revealed two nucleotide substitutions. One substitution (c.398G/C) would nominally change the amino acid sequence (p.S133T) of yak calpastatin. The variant sequence A which carried c.398C was the most common in the yaks tested (95.1%). This is the first report that found yak CAST is variable, and as in pigs, sheep and cattle, this variation may affect animal production traits.
%K CAST
%K genetic variation
%K Yak (Bos grennies)
%K PCR- single strand conformational polymorphism (PCR-SSCP).
%U http://bioinfopublication.org/viewhtml.php?artid=BIA0000209