%0 Journal Article
%T Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
%A Ayyavu Mahesh
%A Devarajan Thangadurai
%A Gabriel Melchias
%J Biological Research
%D 2012
%I Sociedad de Biolog¨Şa de Chile
%X An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
%K Asteraceae
%K Launaea sarmentosa
%K Leaf explant
%K Organogenesis
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200004