%0 Journal Article
%T Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers
%A Ignjatovi£¿-Mici£¿ Dragana
%A Nikoli£¿ Ana
%A Mladenovi£¿-Drini£¿ Sne£¿ana
%A Van£¿etovi£¿ Jelena
%J Genetika
%D 2006
%I Serbian Genetics Society
%R 10.2298/gensr0603227i
%X Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS.
%K CMS
%K maize
%K multiplex PCR
%U http://www.doiserbia.nb.rs/img/doi/0534-0012/2006/0534-00120603227I.pdf