%0 Journal Article %T ※One Step and Two Steps§ freezing methods of dog*s semen in coconut water and ethylene glycol extender M谷todos de congelamento "One Step" e "Two Steps" do s那men de c es, dilu赤do em solu o de 芍gua de coco e etilenoglicol %A D谷bora Silva Godim %A Ana Cristina Nery de Castro %A Marceline Vidal %A Marco Antonio da Rocha Ferreira %J Revista Brasileira de Sa迆de e Produˋˋo Animal %D 2009 %I Universidade Federal da Bahia %X The objective of this study was to verify the influence of One Step and Two Steps freezing methods on dog*s spermatic viability. The experiment was performed using 20 ejaculates from 11 previously selected dogs which presented progressive motility over 70% and vigor equal or superior to 3. The samples were frozen in medium composed by coconut water and ethylene glycol as crioprotectant by the methods, One and Two Steps. After thawing, progressive motility, vigor, live spermatozoa percentage and plasmatic membrane integrity were evaluated. The results showed that there was no difference (P>0.05) in evaluated seminal characteristics. For progressive motility, the results (mean ㊣ standard deviation) were respectively for One and Two Steps, 47.3% ㊣ 16.6% and 50.5% ㊣1 7.4%. As well as, the results for vigor, live spermatozoa percentage, plasmatic membrane integrity were 2.64 ㊣ 0.67 and 2.9 ㊣ 0.74; 52.2% ㊣ 24.16 and 52.2% ㊣ 24.48%; 15.27% ㊣ 10.19% and 23.3% ㊣ 11.6% for One and Two Steps methods, respectively. In conclusion, the One Step and Two Steps techniques can be used as semen cryopreservation methods in dog*s assisted reproduction and there is no difference between the evaluated characteristics of frozen-thawed semen using one or other technique. Objetivou-se, com este estudo, verificar a influ那ncia dos m谷todos de congelamento, One Step e Two Steps, na viabilidade esperm芍tica em c es. Foram utilizados 20 ejaculados de 11 c es, previamente selecionados, que apresentaram motilidade progressiva acima de 70% e vigor igual ou superior a tr那s. As amostras foram congeladas em meio composto de 芍gua de coco e etilenoglicol, pelos m谷todos de One e Two Steps, e avaliadas ap車s descongelamento, quanto 角 motilidade progressiva, vigor, porcentagem de espermatoz車ides vivos e integridade de membrana plasm芍tica. Ap車s o processo de dilui o e congelamento One Step e Two Steps, verificou-se que n o houve diferen a (P>0,05) nas caracter赤sticas seminais, cujos resultados (m谷dia ㊣ desvio padr o) foram, respectivamente, de 47,3% + 16,6% e 50,5% + 17,4% para motilidade progressiva; 2,64 + 0,67 e 2,9 + 0,74 para o vigor; 52,2% + 24,16% e 52,2%+24,48% para porcentagem de espermatoz車ides vivos e de 15,27% + 10,19% e 23,3% + 11,6% para a integridade de membrana plasm芍tica. A aus那ncia de diferen as entre as caracter赤sticas avaliadas para o s那men congelado, permite inferir que as t谷cnicas One Step e Two Steps podem ser utilizadas para a criopreserva o do s那men de c es. %U http://revistas.ufba.br/index.php/rbspa/article/view/1361