%0 Journal Article %T Milk progesterone enzyme-linked immunosorbent assay as a tool to investigate ovarian cyclicity of water buffaloes in relation to body condition score and milk production %A Turgish A Banu %A Mohammed Shamsuddin %A Jayonta Bhattacharjee %A Mohammad F Islam %A Saiful I Khan %A Jalal U Ahmed %J Acta Veterinaria Scandinavica %D 2012 %I BioMed Central %R 10.1186/1751-0147-54-30 %X Progesterone concentrations were measured by an ELISA in milk of 23 postpartum buffaloes in relation to oestrus, pregnancy, body condition score (BCS) and milk production. Two milk samples were taken at 10ˋdays intervals, every month starting from day 30 and continued to day 150 post partum. BCS and milk production were recorded during sample collection. Milk samples from bred buffaloes were collected at Day 0 (day of breeding), Days 10每12 and Days 22每24. Defatted milk was preserved at ˋ80∼C until analysis. Pregnancy was confirmed by palpation per rectum on Days 70每90.Seventeen buffaloes had 47 ovulatory cycles, one to four in each, 13 were detected in oestrus once (28ˋ% oestrus detection rate). Progesterone concentration ≡1ˋng/ml in one of the two 10-day-interval milk samples reflected ovulation and corpus luteum formation. The intervals between calving to first luteal activity and to first detected oestrus varied from 41 to 123ˋdays (nˋ=ˋ17) and 83 to 135 (nˋ=ˋ13) days, respectively. Eight buffaloes were bred in the course of the study and seven were found pregnant. These buffaloes had a progesterone profile of low (<1ˋng/ml), high (≡ 1ˋng/ml) and high (≡ 1ˋng/ml) on Day 0, Days 10每12 and Days 22每24, respectively. Buffaloes cycling later in the postpartum period had fewer missed oestruses (Pˋ<ˋ0.05). Buffaloes with a superior BCS had a shorter calving to oestrus interval and produced more milk (Pˋ<ˋ0.05).Milk progesterone ELISA is a reliable tool for monitoring ovarian cyclicity and good BCS may be an indicator of resuming cyclicity in water buffalo. %K Milk %K Progesterone %K ELISA %K Cyclicity %K Body condition score %K Buffalo %U http://www.actavetscand.com/content/54/1/30/abstract