%0 Journal Article
%T Quantitative determination of xylo-oligosaccharides in xylo-oligosaccharide products with high performance anion-exchange chromatography coupled with pulsed amperometric detection<br>高效阴离子交换色谱-脉冲安培检测法定量测定低聚木糖样品中的低聚木糖
%A FAN Li
%A XU Yong
%A LIAN Zhina
%A YONG Qiang
%A YU Shiyuan
%A <br>范丽
%A 徐勇
%A 连之娜
%A 勇强
%A 余世袁
%J 色谱
%D 2011
%I 
%X A method for the analysis of xylo-oligosaccharides(XOS) in xylo-oligosaccharide products, including xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose, was developed using high performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The retention times of xyloheptaose and xylooctaose were calculated according to the linear relationship between the retention time and the polymerization degree. The separation was performed on a CarboPacTM PA200 column (250 mm×3 mm) with a gradient elution of NaOH-NaOAc as the mobile phase. The calibration curves showed good linearity for the xylo-oligosaccharides in the range of 0.804~8.607 mg/L. The detection limits (LODs) and the quantification limits (LOQs) were 0.064~0.111 mg/L and 0.214~0.371 mg/L, respectively. Under the optimized conditions, the recoveries of xylo-oligosaccharides at three different spiked levels ranged from 84.29%~118.19%, with the relative standard deviations (RSDs, n=3) of 0.44%~14.87%. This method is fast and accurate for the quantitative analysis of the xylo-oligosaccharide products.
%K high performance anion-exchange chromatography (HPAEC)
%K pulsed amperometric detection (PAD)
%K xylo-oligosaccharides<br>高效阴离子交换色谱
%K 脉冲安培检测
%K 低聚木糖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=E63584F4927E5976E11D4C948397B458&yid=9377ED8094509821&vid=771469D9D58C34FF&iid=CA4FD0336C81A37A&sid=80A07035DF96B0C4&eid=46CB27789995047D&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=15