%0 Journal Article
%T Identification and Characterization of an Anti-Fibrotic Benzopyran Compound Isolated from Mangrove-Derived Streptomyces xiamenensis
%A Min-Juan Xu
%A Xiao-Jin Liu
%A Yi-Lei Zhao
%A Dong Liu
%A Zhen-Hao Xu
%A Xiao-Meng Lang
%A Ping Ao
%A Wen-Han Lin
%A Song-Lin Yang
%A Zhi-Gang Zhang
%A Jun Xu
%J Marine Drugs
%D 2012
%I MDPI AG
%R 10.3390/md10030639
%X An anti-fibrotic compound produced by Streptomyces xiamenensis, found in mangrove sediments, was investigated for possible therapeutic effects against fibrosis . The compound, N-[[3,4-dihydro-3 S-hydroxy-2 S-methyl-2-(4¡é R-methyl-3¡é S-pentenyl)- 2H-1-benzopyran-6-yl]carbonyl]-threonine (1), was isolated from crude extracts and its structure, including the absolute configuration was determined by extensive spectroscopic data analyses, Mosher¡¯s method, Marfey¡¯s reagent and quantum mechanical calculations. In terms of biological effects, this compound inhibits the proliferation of human lung fibroblasts (WI26), blocks adhesion of human acute monocytic leukemia cells (THP-1) to a monolayer of WI26 cells, and reduces the contractile capacity of WI26 cells in three-dimensional free-floating collagen gels. Altogether, these data indicate that we have identified a bioactive alkaloid (1) with multiple inhibitory biological effects on lung excessive fibrotic characteristics, that are likely involved in fibrosis, suggesting that this molecule might indeed have therapeutic potential against fibrosis.
%K Streptomyces xiamenensis
%K mangrove
%K benzopyran
%K fibroblast
%K anti-fibrosis
%K anti-contractile capacity
%U http://www.mdpi.com/1660-3397/10/3/639