%0 Journal Article
%T Asymmetric Conversion of 1-Phenyl-1,2-ethanediol by Candida parapsilosis SYB-1<br>全细胞近平滑假丝酵母(Candida parapsilosis)SYB–1不对称转化苯基乙二醇
%A NIE Yao
%A XU Yan
%A <br>聂尧
%A 徐岩
%J 过程工程学报
%D 2003
%I 
%X Optically active 1-phenyl-1,2-ethanediol is an extremely valuable and versatile alcohol used as a chiral building block in organic synthesis. Among the microorganisms which are able to produce S-1-phenyl-1,2-ethanediol from the racemate by asymmetric conversion, Candida parapsilosis SYB-1 was selected as an candidate biocatalyst for the preparation of S-1-phenyl-1,2-ethanediol with the optical purity of 91% e.e. and the yield of 88%. It is found that the optimal conditions for biocatalytic conversion were 5% cells, 8?R,S-1-phenyl-1,2-ethanediol, pH 6.5, temperature 33oC and reaction time 48 h. Under such conditions, the optical purity of S-1-phenyl-1,2-ethanediol was improved from 91% e.e. to 99% e.e. By detecting the intermediate produced in the reaction by GC-MS, it was suggested that S-enantiomer was produced from the intermediate identified as b-hydroxyacetophenone by asymmetric reduction after stereoselective oxidation of R-enantiomer to b-hydroxyacetophenone.
%K microbial stereoinversion
%K 1-phenyl-1
%K 2-ethanediol
%K screen
%K asymmetric conversion<br>微生物法
%K 苯基乙二醇
%K 筛选
%K 不对称转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=2003011A359D772DC5D5CB81F7510000&yid=D43C4A19B2EE3C0A&vid=38B194292C032A66&iid=CA4FD0336C81A37A&sid=E203FB1A272C9DD2&eid=CD775AE9DDBD7B53&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=7&reference_num=0