%0 Journal Article
%T NS-398 induces caspase-dependent, mitochondria-mediated intrinsic apoptosis of hepatoma cells
%A Il Han Song
%A Suk Bae Kim
%A Hyun Duk Shin
%A Ha Yan Kang
%A Eun Young Kim
%J Advances in Bioscience and Biotechnology
%P 649-656
%@ 2156-8502
%D 2012
%I Scientific Research Publishing
%R 10.4236/abb.2012.326084
%X The present study was conducted to investigate whether mitochondrial pathway of apoptosis is involved in cyclooxygenase-2 (COX-2) inhibitor-induced growth inhibition of hepatoma cells. The growth rate and pattern of NS-398 (selective COX-2 inhibitor)-treated Hep3B hepatoma cells were analyzed by microscopic examination, DNA fragmentation gel analysis and flow cytometry followed by the cleavage of down-stream caspase 3 and the release of cytosolic fraction of cytochrome c assessed by Western blot analysis. NS-398 induced the growth inhibition of hepatoma cells depending on the concentration of this COX-2 inhibitor and time sequence. Ladder patterned-DNA fragmentation and cytometric redistribution to sub-G1 phase in cell cycle were revealed in NS-398-induced growth inhibition of hepatoma cells. Cytochrome c was translocated from mitochondria to cytosol in time-dependent manner following NS-398 treatment to hepatoma cells. COX-2 inhibitor induces the growth inhibition of hepatoma cells via caspase-dependent, mitochondria-mediated intrinsic apoptosis pathway. These results strongly suggest the possibility of therapeutic implication of COX-2 inhibitor in HCC.
%K Hepatocellular Carcinoma
%K Cyclooxygenase-2 (COX-2)
%K COX-2 Inhibitor
%K Apoptosis
%K Western Blotting
%K Flow Cytometry
%K DNA
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=23618