%0 Journal Article
%T Nonhost Interaction of Phytophthora sojae and Arabidopsis thaliana and Genetic Analysis of a Susceptible Mutant<br>拟南芥与大豆疫霉菌的非寄主互作及一个感病突变体的遗传分析
%A Qiuping Liu
%A Hua Cao
%A Maojin Yao
%A Ying Ma
%A Binsheng Deng
%A Junli Quan
%A Weixing Shan
%A <br>刘秋萍
%A 曹华
%A 姚茂金
%A 马英
%A 邓斌生
%A 权军利
%A 单卫星
%J 植物学报
%D 2010
%I 
%X Phytophthora sojae and Arabidopsis thaliana were used in this study as a nonhost plant-oomycete interaction system to investigate the genetic basis of nonhost resistance against oomycete pathogens in plants. A collection of more than 40 000 T3 A. thaliana T-DNA mutant plants representing 12 000 independent insertion lines were screened by inoculating detached leaves with P. sojae zoospores, and the susceptible mutant was re-confirmed by pathogen inoculation and cytological characterization. A number of P. sojae-susceptible A. thaliana mutants were successfully obtained, and one of them, mutant 581-51, was shown to be stably susceptible to P. sojae infection. Water-soaked lesions formed on the detached leaves within 3 days, as did oospores and sporangia 4–5 days after inoculation with P. sojae zoospores. Cytological characterization revealed the formation of haustoria-like structures. Southern analysis showed the presence of four T-DNA insertion events in the mutant. Genetic analysis indicated that the susceptibility to infection by the nonhost pathogen P. sojae in the mutant 581-51 was likely controlled by a single recessive gene.
%K Arabidopsis thaliana&prev_q=Arabidopsis thaliana')" href="#">Arabidopsis thaliana" target="_blank">Arabidopsis thaliana')" href="#">Arabidopsis thaliana
%K nonhost disease resistance
%K oomycetes
%K Phytophthora sojae&prev_q=Phytophthora sojae')" href="#"> Phytophthora sojae" target="_blank">Phytophthora sojae')" href="#"> Phytophthora sojae
%K T-DNA insertional mutants<br>拟南芥
%K 非寄主抗病性
%K 卵菌
%K 大豆疫霉菌
%K T-DNA插入突变体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=C5FE317CD31EAFF4BB83BAE60EF8A30D&yid=140ECF96957D60B2&vid=94E7F66E6C42FA23&iid=94C357A881DFC066&sid=E5D85F291CED2DA6&eid=AF4A4411BB448A36&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=0