%0 Journal Article
%T Effective Procedure for Development of EST-SSR Markers Using cDNA Library
%A Kyung A Kim
%A Hee-Cheon Park
%A Jae-Keun Sohn
%A Kyung-Min Kim
%J American Journal of Plant Sciences
%P 1322-1327
%@ 2158-2750
%D 2012
%I Scientific Research Publishing
%R 10.4236/ajps.2012.39159
%X The present study was conducted to develop EST-SSR markers using the cDNA library from rice plant. Total RNA extracted from the leaves of brown plant hopper resistance gene originated from a rice cultivar ¡°Cheongcheong¡± and sensitive rice cultivar ¡°Nakdong¡± were used to synthesize a cDNA library. As a result of analyzing the cDNA library, the 17 EST-SSR primer sets were developed. This study enables to provide effective marker assisted selection (MAS) methods on the selection of white-backed planthopper resistance gene originated from a rice plant more simply, quickly and precisely. Furthermore, using this marker¡¯s advantage of deriving from cDNA, it is possible to identify the white-backed planthopper resistance gene. In addition, this study introduces a technique for construction of a cDNA library safely without using radioactivity.
%K cDNA
%K EST-SSR Markers
%K Radioactivity
%K Rice
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=22909