%0 Journal Article
%T Screen in Saccharomyces cerevisiae for transposon insertion sites able to rescue phenotype of MTM1 deletion mutant using mTn-lacZ/LEU2 transposon library<br>利用酿酒酵母转座子文库筛选MTM1基因缺失表型相关基因
%A Juan Wang
%A Minjie Zhang
%A Yaxue Zeng
%A Ying Cai
%A Chicheng Sun
%A Bing Zhou
%A <br>王娟
%A 张玟洁
%A 曾雅雪
%A 蔡颖
%A 孙驰程
%A 周兵
%J 微生物学报
%D 2010
%I 
%X Objective] MTM1 gene is essential for superoxide dismutase 2 activity and normal mitochondrial functions. MTM1 deletion results in decreased superoxide dismutase 2 activity,impaired mitochondrial functions and growth defect on nonfermentable carbon source. To promote understanding of MTM1 gene,we started a genetic screen for transposon insertions which are able to rescue the growth defect resulting from MTM1 deletion. Methods] Routine screening strategy didn't work because of the irreversible damage cause...
%K Keywords: Saccharomyces cerevisiae
%K mTn-lacZ/LEU2 transposon library
%K MTM1<br>关键词：酵母
%K 转座子文库
%K MTM1
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=369A0EA1AA85C79679E00F76A184A69E&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=CA4FD0336C81A37A&sid=6FBD78E3BAB60869&eid=6DE26652A1045643&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=0