%0 Journal Article
%T Properties of a triphenylmethane dyes decolorization enzyme (TpmD) from Aeromonas hydrophila strain DN322<br>细菌脱色酶TpmD的酶学特性研究
%A REN Sui-zhou
%A GUO Jun
%A CEN Ying-hua
%A SUN Guo-ping
%A <br>任随周
%A 郭俊
%A 王亚丽
%A 岑英华
%A 孙国萍
%J 微生物学报
%D 2006
%I 
%X A novel bacterial enzyme for decolorization of triphenylmethane dyes from Aeromonas hydrophila strain DN322 was purified and named TpmD.The basic properties of this enzyme including molecular weight,isoelectric point Km as well as the optimum temperature and pH were determined and the enzyme was identified as an NADH/NADPH-dependent oxygenase in previous research.Based on previous results,the effect of different inhibitor including Vc,metyrapone,rotenone,antimycin A and NaN-3 as well as the effect of FAD and FMN on the activity of TpmD were measured.The results indicated that the activity of the decolorization enzyme was inhibited by Vc and metyrapone in a concentration-dependent manner,but wasn't inhibited by rotenone,antimycin A and NaN-3.The activity of the decolorization enzyme was not enhanced by addition of FAD or FMN.The solution of the enzyme protein displayed only a single peak at 408nm in the Soret region,a characteristic peak of porphyrin,but did not show the characteristic peak of the cytochrome P450 proteins at 450nm in sodium dithionite(DTN)-reduced enzyme solution after treatment with carbon monoxide.The amino acid sequence of N-terminal of TpmD provided further evidence that the enzyme is an oxygenase.All these results suggest that decolorization enzyme TpmD is a new hemo-containing oxygenase.The decolorization enzyme would be a good material for further research of the enzymological mechanism of triphenylmethane dyes decolorization by bacteria.
%K Decolorization enzyme
%K Aeromonas hydrophila
%K Enzymological properties<br>脱色酶
%K 三苯基甲烷类染料
%K 酶学特性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=28BEA97C651AA6C3&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=94C357A881DFC066&sid=CE504F5B1E192581&eid=4C69616AE50D2DDC&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=22