%0 Journal Article
%T Colloidal gold immunochromatographic strip for rapid detection of melamine<br>三聚氰胺胶体金免疫层析试纸条的研制
%A Yunfei Gong
%A Zonglun Chen
%A Xi Xi
%A Mujie Li
%A Weifen Wang
%A Minzi Wang
%A Yongfei Ying
%A Mingzhou Zhang
%A <br>龚云飞
%A 陈宗伦
%A 奚茜
%A 李沐洁
%A 王唯芬
%A 王旻子
%A 应永飞
%A 张明洲
%J 生物工程学报
%D 2012
%I 
%X To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect melamine residues in dairy products and feed sample. Colloidal gold particles labeled with purified monoclonal antibody against anti-melamine were used as the detector reagent. The MEL-OVA (the conjugate of melamine and ovalbumin) and goat anti-mouse melamine IgG were blotted on the test and control regions of nitrocellulose membrane. The strip was then assembled with sample pad, absorbing pad, and dorsal shield. The limit of detection (LOD) is 50 mg/L. The test trip was applied to detect melamine in milk, milk powder, and animal feeds, with detection limits of 100 mg/L for milk, 100 ng/g for milk powder, 200 ng/g for feeds. Compared to LC-MS/MS, the ICA could be used to screen a large number of dairy products and feed samples for melamine residue.
%K melamine
%K colloidal gold
%K immunochromatographic
%K strip<br>三聚氰胺
%K 胶体金
%K 免疫层析
%K 试纸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B39D47F72CB13A768A1D401A1E7AC395&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=DF92D298D3FF1E6E&sid=3E851F4852A9516C&eid=9124D83E61CF1CD0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0