%0 Journal Article
%T Evaluation of the Hydroxynitrile Lyase Activity in Cell Cultures of Capulin (Prunus serotina)<br>Evaluation of the Hydroxynitrile Lyase Activity in Cell Cultures of Capulin （Prunus serotina）
%A Liliana Hemandez Hector Luna Arturo Navarro-Ocafia Ma Teresa de Jesus Olivera-Flores Ivon Ayala
%A <br>Liliana Hernández
%A Héctor Lun
%A Arturo Navarro-Oca?
%A Ma Teresa de Jesús Olivera-Flores
%A Ivon Ayala
%J 生物工程学报
%D 2008
%I 
%X Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The threeweek old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.
%K cyanohydrins
%K hydroxynitrile lyase
%K Prunus serotina
%K cell culture<br>细胞
%K 裂解酶
%K 活性
%K 柳叶野黑桃
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D8DAA83B93109B2FAFE048FEF2758087&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=DF92D298D3FF1E6E&sid=EB3DE2AA673AE431&eid=B4942BBE94415B36&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14