%0 Journal Article
%T A Simple Cultural Method for Detection of Mycoplasma bovis
%A Nining Hong
%A Qian De-Xing
%A Zhang Deng-Xiang
%A Ran Long-Zhong
%J Journal of Animal and Veterinary Advances
%D 2012
%I Medwell Online
%R 10.3923/javaa.2012.1643.1646
%X Simple method for the detection of Mycoplasma bovis in both liquid and solid cultures was established. Fresh media were easily prepared and dispatched into an eppendorf tube or a Petri dish, a new cultural procedure was followed with the eppendorf tube closed or the Petri dish sealed. Compared to the conventional methods, appropriate inoculating and subculturing operations got easier and a CO2 incubator was not required; moreover, probable contamination by airborne fungi or bacteria could be reduced or eliminated during long-term incubation. Similarly, M. bovis grew normally and could be investigated by Color-Change Units (CCU) enumeration, colony morphology or PCR assay.
%U http://www.medwellonline.net/abstract/?doi=javaa.2012.1643.1646