%0 Journal Article
%T Development of PCR for the Identification of Porcine Circovirus Type 2 (PCV-2) Genotype PCV-2a and PCV-2b
%A Dongsheng He
%A Yanzong Zhao
%A Danping Su
%A Xiaoyun Niu
%J Journal of Animal and Veterinary Advances
%D 2012
%I Medwell Online
%R 10.3923/javaa.2011.2398.2401
%X PCR was developed to evaluate for its ability to simultaneously detect viral infections of swine. Specific primers were designed for each sub-type of porcine circovirus type 2 (PCV-2); porcine circovirus type 2a (PCV-2a) and porcine circovirus type 2b (PCV-2b). Each target produced specific amplicon with a size of 229 bp (PCV-2a) and 785 bp (PCV-2b). The assay was sensitive and specific in detecting the target agent in clinical specimens. In conclusion, the PCR has the potential to be useful for routine molecular diagnosis and epidemiology.
%U http://www.medwellonline.net/abstract/?doi=javaa.2011.2398.2401