%0 Journal Article
%T BC4707 Is a Major Facilitator Superfamily Multidrug Resistance Transport Protein from Bacillus cereus Implicated in Fluoroquinolone Tolerance
%A Roger Simm
%A Aniko V£¿r£¿s
%A Jaakko V. Ekman
%A Marianne S£¿dring
%A Ingerid Nes
%A Jasmin K. Kroeger
%A Massoud Saidijam
%A Kim E. Bettaney
%A Peter J. F. Henderson
%A Mirja Salkinoja-Salonen
%A Anne-Brit Kolst£¿
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0036720
%X Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of the BC4707 protein in Escherichia coli revealed a role in the energized efflux of xenobiotics. Phenotypic analyses after inactivation of the gene bc4707 in Bacillus cereus ATCC14579 suggested a more specific, but modest role in the efflux of norfloxacin. In addition to this, transcriptional analyses showed that BC4707 is also expressed during growth of B. cereus under non-stressful conditions where it may have a role in the normal physiology of the bacteria. Altogether, the results indicate that bc4707, which is part of the core genome of the B. cereus group of bacteria, encodes a multidrug resistance efflux protein that is likely involved in maintaining intracellular homeostasis during growth of the bacteria.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036720