%0 Journal Article
%T 11¦Â Hydroxysteroid Dehydrogenase Type 2 in the Adrenal Gland by Testosterone Withdrawal of Adult Rats
%A Yvetta A. Koeva
%A Mariana V. Bakalska
%A Elisaveta I. Petrova
%A Nina N. Atanassova
%J Folia Medica
%@ 1314-2143
%D 2010
%I 
%R 10.2478/v10153-010-0045-7
%X Aim: 11¦Â hydroxysteroid dehydrogenase (11¦Â HSD) catalyzes the interconversion of glucocorticoids to inert metabolites in man and rodents and plays a crucial role in regulating corticosteroid hormone action. The physiological role and regulation of 11¦Â HSD type 2 in the adrenal gland remains obscure. Therefore, the aim of the present study was to establish the pattern of 11¦Â HSD type 2 expression in rat adrenal gland under conditions of testosterone withdrawal. Material and Methods: We performed immunohistochemical analyses of adrenal gland sections of ethane dimethanesulphonate (EDS)-treated adult rats. Results: In controls, strong positive 11¦Â HSD type 2 signals were detected in the adrenal cortex cells, but not in the medulla. We observed the lowest 11¦Â HSD type 2 expression intensity 7 days after initial treatment with ethane dimethanesulphonate (EDS) followed by progressive increase in the immunoreactivity toward days 14 and 21. Maximal staining intensity of 11¦Â HSD type 2 in the adrenocorticocytes was found by day 35 after EDS treatment. Conclusions: By using the EDS model the present study provides new data about 11¦Â HSD type 2 expression in the adrenal gland under conditions of testosterone withdrawal of adult rats. Our results elucidate further the functional significance of 11¦Â HSD system in rat adrenal gland and the regulatory role of testosterone in its activity.
%K 11¦Â HSD type 2
%K adrenal gland
%K EDS
%U http://versita.metapress.com/content/g450810830300854/?p=248aa998f1844ec18fa83f6a6819b577&pi=4