%0 Journal Article
%T Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts
%A imen Karasu
%A Ahmet Cumao lu
%A Ali Rifat G¨²rpinar
%A Murat Kartal
%A Lucia Kovacikova
%A Ivana Milackova
%A Milan Stefek
%J Interdisciplinary Toxicology
%@ 1337-9569
%D 2012
%I 
%R 10.2478/v10102-012-0003-8
%X The pomegranate, Punica granatum L., has been the subject of current interest as a medicinal agent with wide-ranging therapeutic indications. In the present study, pomegranate ethanolic seed and hull extracts were tested, in comparison with a commercial sample, for the inhibition of aldose reductase, an enzyme involved in the etiology of diabetic complications. In vitro inhibition of rat lens aldose reductase was determined by a conventional method. Pomegranate ethanolic hull extract and commercial pomegranate hull extract exhibited similar aldose reductase inhibitory activity characterized by IC50 values ranging from 3 to 33.3 ¦Ìg/ml. They were more effective than pomegranate ethanolic seed extract with IC50 ranging from 33.3 to 333 ¦Ìg/ml. Antioxidant action of the novel compounds was documented in a DPPH test and in a liposomal membrane model, oxidatively stressed by peroxyl radicals. All the plant extracts showed considerable antioxidant potential in the DPPH assay. Pomegranate ethanolic hull extract and commercial pomegranate hull extract executed similar protective effects on peroxidatively damaged liposomal membranes characterized by 10 < IC50 < 100 ¦Ìg/ml. Pomegranate ethanolic seed extract showed significantly lower antioxidant activity compared to both hull extracts studied. Pomegranate extracts are thus presented as bifunctional agents combining aldose reductase inhibitory action with antioxidant activity and with potential therapeutic use in prevention of diabetic complications.
%K Pomegranate
%K aldose reductase inhibition
%K antioxidant
%K diabetic complications
%U http://versita.metapress.com/content/398n172644603005/?p=20d23cb4dd1a4fa59520c2eb904f8cc8&pi=2