%0 Journal Article
%T Isolation of 5-Hydroxytryptamine from the Venom of the Indian Red Scorpion (Mesobuthus tamulus)
%A Mesobuthus tamulusA.K. Tiwari
%A M.B. Mandal
%A S. Kanoo
%A A.B. Alex
%A A.C. Jariwala and S.B. DeshpandeMBTMBTMBT
%J The Open Toxinology Journal
%D 2008
%I 
%R 10.2174/1875414700801010001]
%X The present study was undertaken to isolate 5-hydroxytryptamine (5-HT) from crude Indian red scorpion (Mesobuthus tamulus; MBT) venom as it has not been reported yet. Therefore, with the help of bioassay (using gastric fundus contractions) and fluorimetric assay methods, the 5-HT content of crude MBT venom (CV) was determined. The concentration of 5-HT in CV by either of the methods was different (117 times). Further, a 5-HT antagonist methysergide partially blocked the CV-induced gastric fundus contractions. Hence, for isolation and determination of 5-HT in crude MBT venom, CV was subjected to Sephadex gel (G 75-125) filtration chromatography. The eluates were screened for optical density (OD) at 280 nm, lethality and gastric fundus contractions. Two peaks of enhanced gastric fundus contractions were observed between 26-70 ml and 90 -110 ml of eluate volumes. As the first peak (between 26-70 ml) eluates exhibited high OD and lethality, further analysis was not undertaken. The second peak (between 90-110 ml) eluates exhibited very low OD and were non-lethal. On bioassay, the 5-HT content of pooled eluates of second peak was 0.0398 ¡À 0.008 ¦Ìg/ml and by fluorimetric assay it was 0.0396 ¡À 0.007 ¦Ìg/ml. The 5-HT concentration of crude MBT venom per unit mass was 0.0175 ¡À 0.0035 ¦Ìg/mg by bioassay and 0.0174 ¡À 0.0031 ¦Ìg/mg by fluorimetric assay. Further, the gastric fundus contractions elicited by second peak eluates were blocked by methysergide (1.0 ¦ÌM). Thus, the second peak eluates of crude MBT venom contain 5-HT and its concentration was about 0.0174 ¦Ì/mg.
%U http://www.benthamscience.com/open/totnj/articles/V001/1TOTNJ.htm