%0 Journal Article %T In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles %A Ying Meng %A Shaoliang Huang %A Jun Min %A Zhongmin Guo %J Science China Life Sciences %@ 1869-1889 %D 2006 %I %R 10.1007/s11427-006-0259-3 %X Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal-or liver-specific mRNA¡ªtransthyretin (TTR), ¦Á1-anti-trypsin (AAT), ¦Á-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1¡À0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies. %K ES cell %K differentiation %K hepatocyte %K coagulation factor %U http://link.springer.com/article/10.1007/s11427-006-0259-3