%0 Journal Article
%T A novel erythroid differentiation related gene EDRF2 inhibited ¦Á-globin gene expression in K562 cells
%A Duncheng Wang
%A Xiaoming Yang
%A Beifen Shen
%J Chinese Science Bulletin
%@ 1861-9541
%D 2002
%I 
%R 10.1360/02tb9093
%X In previous studies, we found that EDRF2 was an erythroid differentiation related factor, whose expression was markedly upregulated during erythroid differentiation. It suggested that this factor played a role in erythropoiesis. By using rapid amplification of cDNA ends technology, we cloned EDRF2 gene 5 ¡ä- and 3 ¡ä-cDNA ends successfully. Transfection and Northern blot analysis demonstrated that EDRF2 inhibited mRNA expression of a-globin gene, while did not regulate ¦Ã-globin gene expression. Gel shift assay confirmed that DNA-binding activity of erythroid specific transcription factors GATA-1, NF-E2 and AP1 was not affected by either forced overexpression or artificial downregulation of EDRF2 gene in K562 cells. However, we detected that the negative regulator of expression of GATA-1 transcription factor was increased in EDRF2 overexpressed K562 cells. These results strongly suggested that EDRF2 was involved in a-globin gene expression and erythroid differentiation and served as a negative regulator of PU.1 transcription factor.
%K globin gene
%K erythroid specific transcription factors
%K leucine zipper
%K RACE
%K EMSA
%U http://link.springer.com/article/10.1360/02tb9093