%0 Journal Article
%T The Leukemia-Associated Fusion Protein MN1-TEL Blocks TEL-Specific Recognition Sequences
%A W. Martijn ter Haar
%A Magda A. Meester-Smoor
%A Karel H. M. van Wely
%A Claudia C. M. M. Schot
%A Marjolein J. F. W. Janssen
%A Bart Geverts
%A Jacqueline Bonten
%A Gerard C. Grosveld
%A Adriaan B. Houtsmuller
%A Ellen C. Zwarthoff
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0046085
%X The leukemia-associated fusion protein MN1-TEL combines the transcription-activating domains of MN1 with the DNA-binding domain of the transcriptional repressor TEL. Quantitative photobleaching experiments revealed that ~20% of GFP-tagged MN1 and TEL is transiently immobilised, likely due to indirect or direct DNA binding, since transcription inhibition abolished immobilisation. Interestingly, ~50% of the MN1-TEL fusion protein was immobile with much longer binding times than unfused MN1 and TEL. MN1-TEL immobilisation was not observed when the TEL DNA-binding domain was disrupted, suggesting that MN1-TEL stably occupies TEL recognition sequences, preventing binding of factors required for proper transcription regulation, which may contribute to leukemogenesis.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0046085