%0 Journal Article %T Detecci¨®n de anticuerpos IgA y PCR como primeras opciones en el diagn¨®stico de infecci¨®n perinatal por el VIH-1 %A Basualdo %A Ma del Carmen %A Moran %A Kathya %A Alc¨¢ntara %A Patricia %A Gonz¨¢lez %A Elizabeth %A Puentes %A Esteban %A Soler %A Carmen %J Salud P¨²blica de M¨¦xico %D 2004 %I Instituto Nacional de Salud P¨²blica %R 10.1590/S0036-36342004000100007 %X objective: to evaluate the sensitivity and specificity of the polymerase chain reaction (pcr), enzyme-linked immunosorbent assay (elisa) and iga-specific immunoblot assays as ancillary methods to diagnose human immunodeficiency virus (hiv-1) perinatal infection. material and methods: a comparative study was conducted between february and october 2001 at the human retrovirus research unit of mexico's national university. ninety infected and 153 non-infected children were included in the study. viral cultures were the gold standard tests. standardized pcr for a conserved region of the gag gene and hiv-specific iga antibody using elisa and immunoblot were used. statistical analysis of results was performed with spss 10.0. results: iga elisa sensitivity and specificity were 61.1% and 90.8%, respectively. immunoblot had a sensitivity of 82.2% and a specificity of 95.4%. pcr had an overall sensitivity of 98.3% and a specificity of 100% with only one false negative result. if both assays were run, the sensitivity increased to 100% and the specificity to 96%. conclusions: a very high sensitivity and specificity is reached when using together pcr and iga immunoblot; these assays are useful for perinatal diagnosis of hiv-1. %K iga %K polymerase chain reaction %K perinatal diagnosis %K hiv. %U http://www.scielosp.org/scielo.php?script=sci_abstract&pid=S0036-36342004000100007&lng=en&nrm=iso&tlng=en