%0 Journal Article
%T Detecci¨®n y tipificaci¨®n de virus del papiloma humano (VPH) mediante PCR- RFLP
%A Quintero Vega
%A Militza
%A Cruz G¨®mez
%A Jhon Fredy
%A Bastidas
%A Marco
%A M¨¢rquez
%A Lusmary
%A Puig Pons
%A Juan
%J Revista de Obstetricia y Ginecolog¨ªa de Venezuela
%D 2008
%I Sociedad de Obstetricia y Ginecolog¨ªa de Venezuela
%X objective: to standardize a pcr-rflp technique for detection and typification of hpv in women with clinical diagnostic of hpv infection. method: descriptive and transversal study with the assessment of 189 samples of the genital area of women that required dna extraction, diagnostic and typification for pcr-rflp molecular technique. setting: facultad de ciencias, laboratorio de biologia y medicina experimental labiomex, universidad de los andes. merida, estado merida, venezuela. results: the pcr-rflp was able to detect and identify the virus. the 16.8 % of the samples presented hpv of high types 16, 31, 33, 35, 56, 59 and 68; the 6.8 % presented hpv of intermediate risk types 51, 53, 58, 61 and 83; and 18 % the low risk types 6, 32, 53, 54, and 81. conclusions: the standardized pcr-rflp technique was appropriate for the diagnostic and typification of hpv in samples from the genital area. the 51.9 % of samples were positive to hpv, been the more common (20 %) the hpv high risk subtype 16, and the low risk hpv 6 the more frequent (23.8%).
%K human papillomavirus
%K cervical infection
%K pcr-rflp
%K cervical cancer.
%U http://www.scielo.org.ve/scielo.php?script=sci_abstract&pid=S0048-77322008000100006&lng=en&nrm=iso&tlng=en