Salt is an important environmental factor that affects wheat growth and development and restricts crop production. Bacillus subtilis QM3 is a strain of antagonistic strains which have biological control function. In this paper, the effects of wheat seed, and soaked treatment by B. subtilis QM3, on germination and antioxidant enzyme activities under salt stress were studied. The results showed that under salt stress, B. subtilis QM3 soaking could increase the germination percentage (GP), germination potential (GT), and germination index (GI) of wheat, and significantly increase the activity of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) (p < 0.05). And high salt concentration had an adverse effect on wheat seed germination. When NaCl concentration were 100, 150, 200 mmol/L, GP, GT, GI decreased to 58.0%, 41%, 61 a/dy, and SOD, POD, CAT decreased to 133.12 g·min, 106.62 g·min, 6.68 g·min. However, the B. subtilis QM3 influenced the variables evaluated. After soaking with B. subtilis QM3, the morphological index GP, GT, GI was significantly increased by 12.99%, 41.18%, and 38.81% at NaCl concentration of 150 mmol/L. Under salt stress (50 mmol/L), B. subtilis QM3 treatment maximized the relative growth rate of CAT activity, which was 87.25%. Similarly, when the NaCl concentration was 150 mol/L, the relative growth rate of SOD activity and POD activity was the greatest, which were 35.81% and 36.57%. It was concluded that B. subtilis QM3 treatment on wheat seeds may be a good option to improve seed germination under salt stress.
Cite this paper
Hu, Q. , Liu, R. and Liu, J. (2019). Effects of Bacillus subtilis QM3 on Germination and Antioxidant Enzymes Activities of Wheat Seeds under Salt Stress. Open Access Library Journal, 6, e5218. doi: http://dx.doi.org/10.4236/oalib.1105218.
Li, R., Shi, F. and Fukuda, K. (2010) Interactive Effects of Salt and Alkali Stresses on Seed Germination, Germination Recovery, and Seedling Growth of a Halophyte Spartina alterniflora (Poaceae). South African Journal of Botany, 76, 380-387. https://doi.org/10.1016/j.sajb.2010.01.004
Hu, Q.-P. and Xu, J.-G. (2011) A Simple Double-Layered Chrome Azurol S agar (SD-CASA) Plateassay to Optimize the Production of Siderophores by a Potential Biocontrol Agent Bacillus. African Journal of Microbiology Research, 25, 4321-4327.
Hao, Y., Wu, H.T., Liu, Y.F. and Hu, Q.P. (2015) Mitigative Effect of Bacillus subtilis QM3 on Root Morphology and Resistance Enzyme Activity of Wheat Root under Lead Stress. Advances in Microbiology, 5, 469-478. https://doi.org/10.4236/aim.2015.56048
Xiao, X., Feng, Q.-Y., Ding, Y., Zhang, S. and Jia, H.-X. (2009) The Effect of Cd on Ultrastructure of Wheat. Procedia Earth and Planetary Science, 1, 1253-1257. https://doi.org/10.1016/j.proeps.2009.09.193
Zhou, L.N., Xia, M.J., Wang, L. and Mao, H. (2016) Toxic Effect of Per?uo-rooctanoic Acid (PFOA) on Germination and Seedling Growth of Wheat (Triticum aestivum L.). Chemosphere, 159, 420-425.https://doi.org/10.1016/j.chemosphere.2016.06.045
Creus, C.M., Sueldo, R.J. and Barassi, C.A. (1997) Shoot Growth and Water Status in Azospirillum-Inoculated Wheat Seedlings Grown under Osmotic and Salt Stresses. Plant Physiology and Bio-chemistry, 35, 939-944.
Xu, L.L. and Ye, M.B. (1989) A Measurement of Peroxidase Activity Using Continuous Recording Method. Journal of Nanjing Agricultural University, 12, 82-83.
Nadeem, S.M., Zahir, Z.A., Naveed, M., Asghar, H.N. and Arshad, M. (2010) Rhizobacteria Capable of Producing ACC-Deaminase May Mitigate Salt Stress in Wheat. Soil Science Society of America Journal, 74, 533-542. https://doi.org/10.2136/sssaj2008.0240
Siddiqui, Md.N., et al. (2017) Impact of Salt-Induced Toxicity on Growth and Yield-Potential of Local Wheat Cultivars: Oxidative Stress and Ion Toxicity Are among the Major Determinants of Salt-Tolerant Capacity. Chemosphere, 187, 385-394. https://doi.org/10.1016/j.chemosphere.2017.08.078
Bahari, A., Pirdashti, H. and Yaghubi, M. (2013) The Effects of Amino Acid Fertilizers Spraying on Photosynthetic Pigments and Antioxidant Enzymes of Wheat (Triticum aestivum L.) under Salinity Stress. International Journal of Agronomy and Plant Production, 4, 787-793.
Elhamid, E.M.A., Sadak, M.Sh. and Tawfik, M.M. (2014) Alleviation of Adverse Effects of Salt Stress in Wheat Cultivars by Foliar Treatment with Antioxidant 2-Changes in Some Biochemical Aspects, Lipid Peroxidation, Antioxidant Enzymes and Amino Acid Contents. Agricultural Sciences, 5, 1269-1280. https://doi.org/10.4236/as.2014.513135