Enrichment and
purification of ursolic acid from Cynomorium
songaricum exacts were studied using five macroporous resins. The static
tests indicated that D101 resin was appropriate and its adsorption data were
well fitted to the Langmuir and Freundlich isotherms. To optimize the
separation process, dynamic adsorption and desorption tests were carried out.
The optimal adsorption parameters were initial concentrations in sample
solution of 3.8 mg/mL,
pH of 6.0,
sample loading amount of 5 BV,
flow rate of 2 BV/h, and temperature of 25?C. The optimal desorption
parameters were 70% ethanol 5 BV, then 80% ethanol 10 BV, and flow rate of 2 BV/h. After one run treatment with AB-8 resin, the
content of total flavonoids in the product increased from 9.83% to
67.8%, and the recovery yield was 84.02%. The results showed that AB-8 resin revealed a good
ability to enrichment total flavonoids from Cynomorium
songaricum, and the method can be referenced for the enrichment of total
flavonoids from other materials.
Cite this paper
Zhang, X. , Hou, W. and Zheng, X. (2018). Separation and Purification of Ursolic Acid from Cynomorium songaricum Exacts with Macroporous Resins. Open Access Library Journal, 5, e4475. doi: http://dx.doi.org/10.4236/oalib.1104475.
Liu, G.D., Chen, G.L., Li, W. and Li, C.X. (2013) Genetic and Phytochemical Diversities of Cynomorium songaricum Rupr. in Northwest China Indicated by ISSR Markers and HPLC-Fingerprinting. Biochemical Systematics and Ecology, 48, 34-41. https://doi.org/10.1016/j.bse.2012.12.004
Ma, L.J., Chen, G.L., Jin, S.W. and Wang, C.X. (2006) The Anti-Aging Effect and the Chemical Consttuents of Cynomorium songaricum Rupr. Acta Horticulturae, 765, 23-30.
Jin, S.W., Chen, G.L., Du, J.J.M., Wang, L.H., Ren, X. and An, T.Y. (2014) Antioxidant Properties and Principal Phenolic Compositions of Cynomorium songaricum Rupr. International Journal of Food Properties, 17, 13-25. https://doi.org/10.1080/10942912.2011.587623
Ma, C.M., Nakamura, N., Miyashiro, H., Hattori, M. and Shimotohno, K. (1999) Inhibitory Effects of Constituents from Cynomorium songari-cum and Related Triterpene Derivatives on HIV-1 Protease. Chemical and Pharmaceutical Bulletin, 47, 141-145.
Ma, L.J., Chen, G.L., Nie, L.S. and Ai, M. (2009) Effect of Cynomorium songaricum Polysaccharide on Telomere Length in Blood and Brain of D-Galactose-Induced Senescence Mice. China Journal of Chinese Materia Medica, 34, 1257-1260.
Zhang, X.R., Jia, Z.P., Li, M.X., Wang, J., Yin, Q., Luo, J.D. and Liu, H.Y. (2008) Study on the Effect of Part III from Cynomorium songaricum on Immu-nosuppressive Mice Induced by Cyclophosphamide. Journal of Chinese Medicinal Materials, 31, 407-409.
Tao, J., Tu, P., Xu, W. and Chen, D. (1999) Studies on Chemical Constituents and Pharmacological Effects of the Stem of Cynomorium songaricum Rupr. Journal of Chinese Medicinal Materials, 24, 292-294.
Zheng, L.L., Wang, D., Li, Y.Y., Peng, H.Y., Yuan, M.Y. and Gao, F. (2014) Ultrasound-Assisted Extraction of Total Flavonoids from Aconitum gymnandrum. Pharmacognosy Magazine, 10, 141-146. https://doi.org/10.4103/0973-1296.127364
Cai, F., Xiao, C., Chen, Q., Ouyang, C., Zhang, R., Wu, J. and Liu, C. (2014) Orthogonal Test Design for Optimizing the Extraction of Total Flavonoids from Flos Pueraria. African Journal of Pharmacy and Pharmacology, 8, 1-8. https://doi.org/10.5897/AJPP2013.3939
Srinivas, K., King, J.W., Monrad, J.K., Howard, L.R. and Zhang, D. (2011) Pressurized Solvent Extraction of Flavonoids from Grape Pomace Utilizing Organic Acid Additives. Italian Journal of Food Science, 23, 90-105.
Bi, W., Tian, M. and Row, K.H. (2013) Evaluation of Alcohol-Based Deep Eutectic Solvent in Extraction and Determination of Flavonoids with Response Surface Methodology Optimization. Journal of Chromatography A, 1285, 22-30. https://doi.org/10.1016/j.chroma.2013.02.041
Yin, H., Zhang, S., Long, L., Yin, H., Tian, X., Luo, X. and He, S. (2013) The Separation of Flavonoids from Pongamia pinnata Using Combination Columns in High-Speed Counter-Current Chromatography with a Three-Phase Solvent System. Journal of Chromatography A, 1315, 80-85. https://doi.org/10.1016/j.chroma.2013.09.010
Dmitrienko, S.G., Stepanova, A.V., Kudrinskaya, V.A. and Apyari, V.V. (2012) Specifics of Separation of Flavonoids by Reverse Phase High Performance Liquid Chromatography on the Luna 5u C18(2) Column. Moscow University Chemistry Bulletin, 67, 254-258.
Goncalves, M.S., Vieira, I.J.C., Oliveira, R.R. and Braz-Filho, R. (2011) Application of Preparative High-Speed Counter-Current Chromatography for the Separation of Two Alkaloids from the Roots of Tabernaemontana catharinensis (Apocynaceae). Molecules, 16, 7480-7487.
https://doi.org/10.3390/molecules16097480
Jiang, Z.G., Du, Q.Z. and Sheng, L.Y. (2009) Separation and Purification of Lentinan by Preparative High Speed Counter Current Chromatography. Chinese Journal of Analytical Chemistry, 37, 412-416.
Shi, S., Zhang, Y., Huang, K., Liu, S. and Zhao, Y. (2008) Application of Preparative High-Speed Counter-Current Chromatography for Separation and Purification of Lignans from Taraxacum mongolicum. Food Chemistry, 108, 402-406. https://doi.org/10.1016/j.foodchem.2007.10.069
Yin, L., Xu, Y., Qi, Y., Han, X., Xu, L., Peng, J. and Sun, C.K. (2010) A Green and Efficient Protocol for Industrial-Scale Preparation of Dioscin from Dioscorea nipponica Makino by Two-Step Macroporous Resin Column Chromatography. Chemical Engineering Journal, 165, 281-289. https://doi.org/10.1016/j.cej.2010.09.045
Sun, P.C., Liu, Y., Yi, Y.T., Li, H.J., Fan, P. and Xia, C.H. (2015) Preliminary Enrichment and Separation of Chlorogenic Acid from Helianthus tuberosus L. Leaves Extract by Macroporous Resins. Food Chem-istry, 168, 55-62. https://doi.org/10.1016/j.foodchem.2014.07.038
Wang, C., Chao, Z., Wu, X., Sun, W. and Ito, Y. (2014) En-richment and Purification of Pedunculoside and Syringin from the Barks of Ilex rotunda with Macroporous Resins. Journal of Liquid Chromatography & Related Technologies, 37, 572-587. https://doi.org/10.1080/10826076.2012.749499
Kim, E.J., Lee, H.J., Kim, H.J., Nam, H.S., Lee, M.K., Kim, H.Y. and Kim, H.Y. (2005) Comparison of Colorimetric Methods for the Determination of Flavonoid in Propolis Extract Products. Korean Journal of Food Science and Technology, 37, 918-921.
Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C. (2002) Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, 10, 178-182.
Afsar, M.Z., Hoque, S. and Osman, K.T. (2012) A Comparison of the Langmuir, Freundlich and Temkin Equations to Describe Phosphate Sorption Characteristics of Some Representative Soils of Bangladesh. International Journal of Soil Science, 7, 91-99. https://doi.org/10.3923/ijss.2012.91.99
Tellan, A.C. and Owalude, S.O. (2007) Some Langmuir and Freundlich Parameters of Adsorption Studies of Chlorpheniramine Maleate. Research Journal of Applied Sciences, 2, 875-878.
Xiao, F. and Pignatello, J.J. (2014) Effect of Adsorption Nonlinearity on the pH-Adsorption Profile of Ionizable Organic Compounds. Langmuir, 30, 1994-2001. https://doi.org/10.1021/la403859u
Zhang, Y., Li, S.F., Wu, X.W. and Zhao, X. (2007) Macroporous Resin Adsorption for Purification of Flavonoids in Houttuynia cordata Thunb. Chinese Journal of Chemical Engineering, 15, 872-876. https://doi.org/10.1016/S1004-9541(08)60017-8
Langmuir, I. (1918) Adsorption of Gases on Plain Surface of Glass, Mica and Platinum. Journal of the American Chemical Society, 40, 1361-1403. https://doi.org/10.1021/ja02242a004
Zhao, Z.Y., Dong, L.L., Wu, Y.L. and Lin, F. (2011) Preliminary Separation and Purification of Rutin and Quercetin from Euonymus alatus (Thunb.) Siebold Extracts by Macroporous Resins. Food and Bioproducts Processing, 89, 266-272. https://doi.org/10.1016/j.fbp.2010.11.001
Zhao, H., Wang, J., Jia, J., Liu, J., Ling, X.Q. and Lu, D.Q. (2010) Enrichment and Purification of Total Chlorogenic Acids from Tobacco Waste Extract with Macroporous Resins. Separation Science and Technology, 45, 794-800. https://doi.org/10.1080/01496390903566713