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Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua

DOI: 10.4236/oalib.1103309, PP. 1-16

Subject Areas: Infectious Diseases, Immunology

Keywords: Dengue Infections, IgM, IgA, IgE, NS1 Protein

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Abstract

Latest estimates suggest 390 million infections of dengue occur each year, of which 100 million results in symptomatic disease. The new clinical classification proposed by WHO-TRD 2009 simplifies dengue fever (DF) into dengue (D) and severe dengue (SD) with an intermediary group of “dengue with warning signs” (DWS). Early, sensitive and specific diagnosis of dengue is needed for appropriate patient management as well as for early epidemic detection. Virological and serological markers in sera from dengue confirmed patients from Venezuela and Nicaragua were evaluated. A total of 232 sera collected in the acute (days 1 to 5 of fever onset) and convalescent (days 7 to 14) phases of illness were tested by Platelia NS1 antigen ELISA and IgM, IgA and IgE capture ELISA methods. NS1 was detected with highest OD values in the acute phase for primary and secondary cases. IgM was detected in primary and secondary groups but the highest OD figures were observed after defervescence day. IgA and IgE detection were almost absent for primary cases, contrary to secondary cases which showed higher values but after defervescence day. NS1 could be used as effective diagnostic marker in the acute phase of illness for primary and secondary dengue infections. IgM detection is still an invaluable tool for routine dengue diagnosis but delay. IgA detection could define secondary infection in early phase of illness. IgA/IgE could be evaluated as prognostic markers associated to SD cases.

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Ruiz, D. , Vázquez, S. , Villegas, E. , Balmaseda, A. , Pupo, M. , Alvarez, M. , Rosario, D. , Bendezu, H. , Calzada, N. and Guzman, M. G. (2017). Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua. Open Access Library Journal, 4, e3309. doi: http://dx.doi.org/10.4236/oalib.1103309.

References

[1]  Yacoub, S., Mongkolsapaya, J. and Screaton, G. (2016) Recent Advances in Understanding Dengue. F1000Research, 5, F1000.
[2]  Guzman, M.G., Halstead, S.B., Artsob, H., Buchy, P., Farrar, J., Gubler, D.J., Hunsperger, E., Kroeger, A., Margolis, H.S., Martinez, E., Nathan, M.B., Pelegrino, J.L., Simmons, C., Yoksan, S. and Peeling, R.W. (2010) Dengue: A Continuing Global Threat. Nature Reviews Microbiology, 8, S7-S16.
https://doi.org/10.1038/nrmicro2460
[3]  Halstead, S.B. and Cohen, S.N. (2015) Dengue Hemorrhagic Fever at 60 Years: Early Evolution of Concepts of Causation and Treatment. Microbiology and Molecular Biology Reviews, 79, 281-291.
https://doi.org/10.1128/MMBR.00009-15
[4]  Guzman, M.G. and Kouri, G. (1996) Advances in Dengue Diagnosis. Clinical and Diagnostic Laboratory Immunology, 3, 621-627.
[5]  WHO (1997) Dengue Hemorrhagic Fever. Diagnosis, Treatment, Prevention and Control. Geneva, 1-84.
[6]  Groen, J., Koraka, P., Velzing, J., Copra, C. and Osterhaus, A.D. (2000) Evaluation of Six Immunoassays for Detection of Dengue Virus-Specific Immunoglobulin M and G Antibodies. Clinical and Diagnostic Laboratory Immunology, 7, 867-871.
[7]  Muller, D.A. and Young, P.R. (2013) The Flavivirus NS1 Protein: Molecular and Structural Biology, Immunology, Role in Pathogenesis and Application as a Diagnostic Biomarker. Antiviral Research, 98, 192-208.
[8]  Tricou, V., Vu, H.T., Quynh, N.V., Nguyen, C.V., Tran, H.T., Farrar, J., Wills, B. and Simmons, C.P. (2010) Comparison of Two Dengue NS1 Rapid Tests for Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses. BMC Infectious Diseases, 10, 142.
https://doi.org/10.1186/1471-2334-10-142
[9]  De Decker, S., Vray, M., Sistek, V., Labeau, B., Enfissi, A., Rousset, D. and Matheus, S. (2015) Evaluation of the Diagnostic Accuracy of a New Dengue IgA Capture Assay (Platelia Dengue IgA Capture, Bio-Rad) for Dengue Infection Detection. PLoS Neglected Tropical Diseases, 9, e0003596.
https://doi.org/10.1371/journal.pntd.0003596
[10]  Koraka, P., Suharti, C., Setiati, T.E., Mairuhu, A.T., Van Gorp, E., Hack, C.E., Juffrie, M., Sutaryo, J., Van Der Meer, G.M., Groen, J. and Osterhaus, A.D. (2001) Kinetics of Dengue Virus-Specific Serum Immunoglobulin Classes and Subclasses Correlate with Clinical Outcome Of Infection. Journal of Clinical Microbiology, 39, 4332-4338.
https://doi.org/10.1128/JCM.39.12.4332-4338.2001
[11]  Guzman, M.G., Jaenisch, T., Gaczkowski, R., Ty Hang, V.T., Sekaran, S.D., Kroeger, A., Vazquez, S., Ruiz, D., Martinez, E., Mercado, J.C., Balmaseda, A., Harris, E., Dimano, E., Leano, P.S., Yoksan, S., Villegas, E., Benduzu, H., Villalobos, I., Farrar, J. and Simmons, C.P. (2010) Multi-Country Evaluation of the Sensitivity and Specificity of Two Commercially-Available NS1 ELISA Assays for Dengue Diagnosis. PLoS Neglected Tropical Diseases, 4, e811.
[12]  Lanciotti, R.S., Calisher, C.H., Gubler, D.J., Chang, G.J. and Vorndam, A.V. (1992) Rapid Detection and Typing of Dengue Viruses from Clinical Samples by Using Reverse Transcriptase-Polymerase Chain Reaction. Journal of Clinical Microbiology, 30, 545-551.
[13]  Vazquez, S., Perez, A.B., Ruiz, D., Rodriguez, R., Pupo, M., Calzada, N., González, L., González, D., Castro, O., Serrano, T. and Guzmán, M.G. (2005) Serological Markers during Dengue 3 Primary and Secondary Infections. Journal of Clinical Virology, 33, 132-137.
https://doi.org/10.1016/j.jcv.2004.10.013
[14]  Vazquez, S., Acosta, N., Ruiz, D., Calzada, N., Alvarez, A.M. and Guzman, M.G. (2009) Immunoglobulin G Antibody Response in Children and Adults with Acute Dengue 3 Infection. Journal of Virological Methods, 159, 6-9.
[15]  WHO (2013) Dengue and Severe Dengue. Media Centre, Updated September. Fact Sheet N 117.
[16]  WHO/TDR (2009) Dengue Case Classification. In: Dengue, Guidelines for Diagnosis Treatment, Prevention and Control, Geneva, 1-146.
[17]  Clark, D.H. and Casals, J. (1958) Techniques for Hem-Agglutination-Inhibition with Arthropod-Borne Viruses. American Journal of Tropical Medicine and Hygiene, 7, 561-573.
[18]  Gonzalez, D., Castro, O., Perez, J., Martinez, E., Vazquez, S., Rosario, D., Cancio, R. and Guzman, M.G. (2005) Classical Dengue Hemorrhagic Fever Resulting from Two Dengue Infections Spaced 20 Years or More Apart: Havana, Dengue 3 Epidemic, 2001-2002. International Journal of Infectious Diseases, 9, 280-285.
https://doi.org/10.1016/j.ijid.2004.07.012
[19]  Gutsche, I., Coulibaly, F., Voss, J.E., Salmon, J., d’Alayer, J., Ermonval, M., Larquet, E., Charneau, P., Krey, T., Megret, F., Guittet, E., Rey, F.A. and Flamand, M. (2011) Secreted Dengue Virus Nonstructural Protein NS1 Is an Atypical Barrel-Shaped High-Density Lipoprotein. Proceedings of the National Academy of Sciences of the United States of America, 108, 8003-8008.
https://doi.org/10.1073/pnas.1017338108
[20]  Shan, X., Wang, X., Yuan, Q., Zheng, Y., Zhang, H., Wu, Y. and Yang, J. (2015) Evaluation of the Diagnostic Accuracy of Nonstructural Protein 1 Ag-Based Tests for Dengue Virus in Asian Population: A Meta-Analysis. BMC Infectious Diseases, 15, 360.
https://doi.org/10.1186/s12879-015-1088-4
[21]  Zhang, H., Li, W., Wang, J., Peng, H., Che, X., Chen, X. and Zhou, Y. (2014) NS1-Based Tests with Diagnostic Utility for Confirming Dengue Infection: A Meta-Analysis. International Journal of Infectious Diseases, 26, 57-66.
https://doi.org/10.1016/j.ijid.2014.02.002
[22]  Hang, V.T., Nguyet, N.M., Trung, D.T., Tricou, V., Yoksan, S., Dung, N.M., Van Ngoc, T., Hien, T.T., Farrar, J., Wills, B. and Simmons, C.P. (2009) Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses. PLoS Neglected Tropical Diseases, 3, e360.
https://doi.org/10.1371/journal.pntd.0000360
[23]  Tricou, V., Minh, N.N., Farrar, J., Tran, H.T. and Simmons, C.P. (2011) Kinetics of Viremia and NS1 Antigenemia Are Shaped by Immune Status and Virus Serotype in Adults with Dengue. PLoS Neglected Tropical Diseases, 5, e1309.
https://doi.org/10.1371/journal.pntd.0001309
[24]  Alcon, S., Talarmin, A., Debruyne, M., Falconar, A., Deubel, V. and Flamand, M. (2002) Enzyme-Linked Immunosorbent Assay Specific to Dengue Virus Type 1 Nonstructural Protein NS1 Reveals Circulation of the Antigen in the Blood during the Acute Phase of Disease in Patients Experiencing Primary or Secondary Infections. Journal of Clinical Microbiology, 40, 376-381.
https://doi.org/10.1128/JCM.40.02.376-381.2002
[25]  Shu, P.Y., Chen, L.K., Chang, S.F., Yueh, Y.Y., Chow, L., Chien, L.J., Chin, C., Lin, T.H. and Huang, J.H. (2003) Comparison of Capture Immunoglobulin M (IgM) and IgG Enzyme-Linked Immunosorbent Assay (ELISA) and Nonstructural Protein NS1 Serotype-Specific IgG ELISA for Differentiation of Primary and Secondary Dengue Virus Infections. Clinical and Diagnostic Laboratory Immunology, 10, 622-630.
[26]  Dussart, P., Labeau, B,. Lagathu, G., Louis, P., Nunes, M.R., Rodrigues, S.G., Storck-Herrmann, C., Cesaire, R., Morvan, J., Flamand, M. and Baril, L. (2006) Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum. Clinical and Vaccine Immunology, 13, 1185-1189.
https://doi.org/10.1128/CVI.00229-06
[27]  Xu, H., Di, B., Pan, Y.X., Qiu, L.W., Wang, Y.D., Hao, W., He, L.J., Yuen, K.Y. and Che, X.Y. (2006) Serotype 1-Specific Monoclonal Antibody-Based Antigen Capture Immunoassay for Detection of Circulating Nonstructural Protein NS1: Implications for Early Diagnosis and Serotyping of Dengue Virus Infections. Journal of Clinical Microbiology, 44, 2872-2878.
https://doi.org/10.1128/JCM.00777-06
[28]  Hu, D., Di, B., Ding, X., Wang, Y., Chen, Y., Pan, Y., Wen, K., Wang, M. and Che, X. (2011) Kinetics of Non-Structural Protein 1, IgM and IgG Antibodies in Dengue Type 1 Primary Infection. Virology Journal, 8, 47.
https://doi.org/10.1186/1743-422X-8-47
[29]  Guzman, M.G. and Kouri, G. (2004) Dengue Diagnosis, Advances and Challenges. International Journal of Infectious Diseases, 8, 69-80.
https://doi.org/10.1016/j.ijid.2003.03.003
[30]  Summers, P.L., Eckels, K.H., Dalrymple, J.M., Scott, R.M. and Boyd, V.A. (1984) Antibody Response to Dengue-2 Vaccine Measured by Two Different Radioimmunoassay Methods. Journal of Clinical Microbiology, 19, 651-659.
[31]  Innis, B.L., Nisalak, A., Nimmannitya, S., Kusalerdchariya, S., Chongswasdi, V., Suntayakorn, S., Puttisri, P. and Hoke, C.H. (1989) An Enzyme-Linked Immunosorbent Assay to Characterize Dengue Infections Where Dengue and Japanese Encephalitis Co-Circulate. The American Journal of Tropical Medicine and Hygiene, 40, 418-427.
[32]  Nogueira, R.M., Miagostovich, M.P., Cavalcanti, S.M., Marzochi, K.B. and Schatzmayr, H.G. (1992) Levels of IgM Antibodies against Dengue Virus in Rio de Janeiro, Brazil. Research in Virology, 143, 423-427.
[33]  Klein, U., Goossens, T., Fischer, M., Kanzler, H., Braeuninger, A., Rajewsky, K. and Kuppers, R. (1998) Somatic Hypermutation in Normal and Transformed Human B Cells. Immunological Reviews, 162, 261-280.
https://doi.org/10.1111/j.1600-065X.1998.tb01447.x
[34]  Agematsu, K., Hokibara, S., Nagumo, H. and Komiyama, A. (2000) CD27: A Memory B-Cell Marker. Trends in Immunology, 21, 204-206.
https://doi.org/10.1016/s0167-5699(00)01605-4
[35]  Kruetzmann, S., Rosado, R., Weber, H., Germing, U., Tournilhac, O., Peter, H., Berner, R., Peters, A., Boehm, T., Plebani, A., Quinti, I. and Carsetti, R. (2003) Human Immunoglobulin M Memory B Cells Controlling Streptococcus pneumonia Infections Are Generated in the Spleen. Journal of Experimental Medicine, 197, 939-945.
https://doi.org/10.1084/jem.20022020
[36]  Weller, S., Braun, M.C., Tan, B.K., Rosenwald, A., Cordier, C., Conley, M.E., Plebani, A., Kumararatne, D.S., Bonnet, D., Tournilhac, O., Tchernia, G., Steiniger, B., Staudt, L.M., Casanova, J.-L., Reynaud, C.-A. and Weill, J.-C. (2004) Human Blood IgM “Memory” B Cells Are Circulating Splenic Marginal Zone B Cells Harboring a Prediversified Immunoglobulin Repertoire. Blood, 104, 3647-3654.
https://doi.org/10.1182/blood-2004-01-0346
[37]  White, H. and Gray, D. (2000) Analysis of Immunoglobulin (Ig) Isotype Diversity and IgM/D Memory in the Response to Phenyl-Oxazolone. Journal of Experimental Medicine, 191, 2209-2219.
https://doi.org/10.1084/jem.191.12.2209
[38]  Shi, Y., Agematsu, K., Ochs, H. and Suganea, K. (2003) Functional Analysis of Human Memory B-Cell Subpopulations: IgD CD27 B Cells Are Crucial in Secondary Immune Response by Producing High Affinity IgM. Clinical Immunology, 108, 128-137.
[39]  Naz, A., Zahid, D., Murky, S.N., Nadeem, M., Sil, B.K. and Shamsi, T.S. (2014) Evaluation of Efficacy of Various Immunochromatographic Rapid Tests for Dengue Diagnosis. Pakistan Journal of Medical Sciences, 30, 166-171.
[40]  Balmaseda, A., Guzman, M.G., Hammond, S., Robleto, G., Flores, C., Tellez, Y., Videa, E., Saborio, S., Perez, L., Sandoval, E., Rodriguez, Y. and Harris, E. (2003) Diagnosis of Dengue Virus Infection by Detection Of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva. Clinical and Diagnostic Laboratory Immunology, 10, 317-322.
[41]  Nawa, M., Takasaki, T., Ito, M., Inoue, S., Morita, K. and Kurane, I. (2005) Immunoglobulin A Antibody Responses in Dengue Patients: A Useful Marker for Serodiagnosis of Dengue Virus Infection. Clinical and Diagnostic Laboratory Immunology, 12, 1235-1237.
[42]  Blacksell, S.D., Jarman, R.G., Gibbons, R.V., Tanganuchitcharnchai, A., Mammen Jr., M.P., Nisalak, A., Kalayanarooj, S., Bailey, M.S., Premaratna, R., de Silva, H.J., Day, N.P. and Lalloo, D.G. (2012) Comparison of Seven Commercial Antigen and Antibody Enzyme-Linked Immunosorbent Assays for Detection of Acute Dengue Infection. Clinical and Vaccine Immunology, 19, 804-810.
https://doi.org/10.1128/CVI.05717-11
[43]  Pérez, A.B., García, G., Sierra, B., Alvarez, M., Vázquez, S., Cabrera, M.V., Rodriguez, R., Rosario, D., Martinez, E., Denny, T. and Guzmán, M.G. (2004) IL-10 Levels in Dengue Patients: Some Findings from the Exceptional Epidemiological Conditions in Cuba. Journal of Medical Virology, 73, 230-234.
https://doi.org/10.1002/jmv.20080
[44]  Sierra, B., Perez, A.B., Vogt, K., Garcia, G., Schmolke, K., Aguirre, E., Alvarez, M., Kern, F., Kouri, G., Volk, H.D. and Guzman, M.G. (2010) Secondary Heterologous Dengue Infection Risk: Disequilibrium between Immune Regulation and Inflammation? Cellular Immunology, 262, 134-140.
[45]  Pate, M., Smith, J., Chi, D. and Krishnaswamy, G. (2010) Regulation and Dysregulation of Immunoglobulin E: A Molecular and Clinical Perspective. Clinical and Molecular Allergy, 8, 3.
https://doi.org/10.1186/1476-7961-8-3
[46]  Alexeyev, O.A., Ahlm, C., Billheden, J., Settergren, B., Wadell, G. and Juto, P. (1994) Elevated Levels of Total and Puumala Virus-Specific Immunoglobulin E in the Scandinavian Type of Hemorrhagic Fever with Renal Syndrome. Clinical and Diagnostic Laboratory Immunology, 1, 269-272.
[47]  Miguez-Burbano, M.J., Jaramillo, C.A., Palmer, C.J., Shor-Posner, G., Velasquez, L.S., Lai, H. and Baum, M.K. (1999) Total Immunoglobulin E Levels and Dengue Infection on San Andres Island, Colombia. Clinical and Diagnostic Laboratory Immunology, 6, 624-626.
[48]  Koraka, P., Murgue, B., Deparis, X., Setiati, T.E., Suharti, C., van Gorp, E.C., Hack, C.E., Osterhaus, A.D. and Groen, J. (2003) Elevated Levels of Total and Dengue Virus-Specific Immunoglobulin E in Patients with Varying Disease Severity. Journal of Medical Virology, 70, 91-98.
https://doi.org/10.1002/jmv.10358
[49]  Stone, K., Prussin, C. and Metcalfe, D. (2010) IgE, Mast Cells, Basophils, and Eosinophils. Journal of Allergy and Clinical Immunology, 125, S73-S80.
https://doi.org/10.1016/j.jaci.2009.11.017
[50]  Vercelli, D. (2009) Immunobiology of IgE. In: Adkinson, N., Bochner, B.S., Busse, W.W., Holgate, S.T., Lemanske, R.F. and Simons, F.E.R., Eds., Middletons Allergy: Principles and Practice, 7th Edition, Mosby Elsevier, St Louis.
[51]  Kouri, G.P., Guzman, M.G. and Bravo, J.R. (1987) Why Dengue Haemorrhagic Fever in Cuba? 2. An Integral Analysis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 81, 821-823.
https://doi.org/10.1016/0035-9203(87)90042-3
[52]  Guzman, M.G. and Kouri, G. (2008) Dengue Haemorrhagic Fever Integral Hypothesis: Confirming Observations, 1987-2007. Transactions of the Royal Society of Tropical Medicine and Hygiene, 102, 522-523.
https://doi.org/10.1016/j.trstmh.2008.03.001

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