We previously proposed the existence of DNA to DNA transcription in
eukaryotic cells, but the mechanism by which single-stranded DNA (ssDNA)
transcript is produced and released from the genome remains unknown. We once
speculated that the mechanism of DNA to DNA transcription might be similar to
that of DNA to RNA transcription, but now we propose that endonuclease dependent
transcript cutout may be a possible mechanism of DNA to DNA transcription, in
which a copy of ssDNA fragment (transcript) between two nicks produced by nicking
endonuclease is released from double-stranded DNA (dsDNA) region in the genome
by an unknown ssDNA fragment releasing enzyme. The gap in the dsDNA will be
filled through DNA repair mechanism. Occasionally, multiple copies of ssDNA
transcripts could be produced through multiple rounds of cutout- repair-cutout
cycle.
Cite this paper
Li, G. (2016). A Possible Mechanism of DNA to DNA Transcription in Eukaryotic Cells: Endonuclease Dependent Transcript Cutout. Open Access Library Journal, 3, e2758. doi: http://dx.doi.org/10.4236/oalib.1102758.
Li, G.D. (2016) Certain Amplified Genomic-DNA
Fragments (AGFs) May Be Involved in Cell Cycle Progression and Chloroquine Is
Found to Induce the Production of Cell-Cycle-Associated AGFs (CAGFs) in Plasmodium falciparum. Open Access Library Journal, 3, e2447. http://dx.doi.org/10.4236/oalib.1102447
Smith, N.G., Brandstrom, M. and Ellegren, H. (2004) Evidence for Turnover of Functional
Noncoding DNA in Mammalian Genome Evolution. Genomics, 84, 806-813. http://dx.doi.org/10.1016/j.ygeno.2004.07.012
Clause, A.R. and Capaldi,
E.A. (2006) Caudal Autotomy and Regeneration in Lizards. Journal of Experimental Zoology Part A: Comparative Experimental Biology, 305, 965-973.
Nur-E-Kamal, A., Li, T.K., Zhang, A., Qi, H., Hars, E.S. and Liu, L.F. (2003) Single-Stranded DNA
Induces Ataxia Telangiectasia Mutant (ATM)/p53 Dependent DNA Damage and
Apoptotic Signals. The Journal of Biological
Chemistry, 278, 12475-12481. http://dx.doi.org/10.1074/jbc.M212915200
Hanania, N., Schaool, D., Poncy, C., Tapiero, H. and
Harel, J. (1977) Isolation of Single Stranded Transcription Sites from Human
Nuclear DNA. Cell Biology International
Reports, 1, 309-315. http://dx.doi.org/10.1016/0309-1651(77)90060-1
Hanania, N., Shaool, D. and Harel, J. (1982) Isolation of
a Mouse DNA Fraction Which Encodes More Informational Than Non Informational RNA
Sequences. Molecular Biology Reports,
8, 91-96. http://dx.doi.org/10.1007/BF00778510
Leibovitch, S.A., Tapiero, H. and Harel, J. (1977) Single-Stranded DNA from
Oncornavirus-Infected Cells Enriched in Virus-Specific DNA Sequences. Proceedings of the National Academy of
Sciences of the USA, 74, 3720-3724. http://dx.doi.org/10.1073/pnas.74.9.3720
Leibovitch, S.A. and Harel, J. (1978) Active DNA
Transcription Sites Released from the Genome of Normal Embryonic Chicken Cells. Nucleic Acids Research, 5, 777-787. http://dx.doi.org/10.1093/nar/5.3.777
Leibovitch, S.A., Tichonicky, L., Kruh, J. and Harel, J. (1981) Single-Strandedness of
the Majority of DNA Sequences Complementary to mRNA-Coding Sites Isolated from
Rat Hepatoma Tissue Cultured Cells. Experimental
Cell Research, 133, 181-189. http://dx.doi.org/10.1016/0014-4827(81)90368-2
Collins, J.M. (1977) Deoxyribonucleic Acid Structure in
Human Diploid Fibroblasts Stimulated to Proliferate. Journal of Biological Chemistry, 252, 141-147.
Wanka, F., Brouns, R.M.,
Aelen, J.M., Eygensteyn, A. and Eygensteyn, J.
(1977) The Origin of Nascent Single- Stranded DNA Extracted
from Mammalian Cells. Nucleic Acids
Research, 4, 2083-2097. http://dx.doi.org/10.1093/nar/4.6.2083
Bjursell, G., Gussander,
E. and Lindahl, T. (1979) Long Regions of Single-Stranded
DNA in Human Cells. Nature, 280, 420-423. http://dx.doi.org/10.1038/280420a0