Specifics of Platelet Hemostasis in Children with Chronic Diseases of Lungs

Study of platelet aggregation function can help early diagnostics of microvasculature’s disorders. Purpose of the study is evaluation of changes of platenet aggregation activity in children with chronic inflammation. Materials and methods: 85 children examined, of whom 45 children with cystic fibrosis (mixed form) aged 8 months to 17 years. The platelet aggregation in children’s whole blood was studied using aggregometer “Multiplate” (VerumDiagnostica, Germany). The following agents were used as inducer of aggregation: adenosine diphosphoric acid solution (ADP), soluble Thrombin Receptor Activator for Peptide 6 (TRAP-test) and arachidonic acid (ASPI-test). C-reactive protein (SRP) in the blood serum was measured by turbidimetric method using biochemical analyzer UniCelDxC 600 (BD, USA). Immunochemiluminescent method was used to determine the level of the main pro-inflammatory cytokine IL-6 (analyzer Accesses 2, BD, USA). Results: changes in the aggregation of platelets can be expressed by platelet activation followed by hyper-aggregation and thrombi formation on the one hand, and disorder of arachidonic acid and ADP metabolism on the other hand that leads to the development of hemorrhagic complications. A necessary condition of the therapy is a presence of antiplatelets and calcic drugs, which help to warn complications in a form of thrombosis and hemorrhages.