Artemisia annua is the source of artemisinin, an antimalarial drug which is effective
against multidrug-resistant strains of plasmodium, the malarial parasite.
Malaria has serious effects on morbidity and mortality thus negatively
impacting on agricultural production and food security. Although artemisinin
has been found to be a useful medicine; its production is very low in comparison
with what is actually needed to treat the worldwide threat of malaria. On the
other hand, the lower content (0.01% - 0.8%, dry weight) of artemisinin found
in leaves and flowers of A. annua has seriously limited its
commercialization. Currently there are only two varieties of A. annua present in Kenya; hence there
is a need to increase its diversity. The objective of the current study was to
determine the effect of mutation on agronomical traits and artemisinin production
by parents and mutant (M2) A.
annua plants in Kenya Agricultural
and Livestock Research Organisation (KALRO) Njoro and the University of Eldoret
(UoE). Seeds of two varieties of Artemesia
annua varartemis and varanamed were sent to Vienna Austria for irradiation at the International Atomic Energy
Agency (IAEA) at a dosage of 150 gray. The M1 seeds were multiplied
at the University of Eldoret farm. The harvested seeds were planted in
replicate at the University of Eldoret and Njoro (KALRO). The results showed
that mutation had significant effect on agronomical traits (P-value < 0.001).
Mutant varieties and lines showed wide variation in terms of agronomical traits
(crown length, stem length, plant height and stem diameter) and yield of
artemisinin. The following lines showed superiority in artemisinin production;
Artemis line 1, 2 and 9 with an average mean of 58.843, 58.393 and 69.192 μg/l,
respectively while Anamed line 2, 3, 5 and 8 with an average of 56.268, 51.704,
53.557 and 54.448 μg/l, respectively. The effect of mutation on both
agronomical traits and production of the artemisinin content appeared to occur
randomly and was also dependent on environmental factors in the different
ecological zones. Higher means in agronomical traits was observed in UoE while
production of artemisinin content was enhanced in Njoro. The artemisinin yield
in A. annua crops was negatively
correlated with leaf traits, shoot and stem characteristics. Leaf traits had
positive correlations with shoot and stem characteristics. It is recommended
that superior lines be advanced in generations for further stability and
evaluation of its efficacy in treatment of malaria.
Cite this paper
Raymond, M. , Miriam, K. , Oliver, K. , Edwin, M. and Stephen, K. (2015). Enhancement of Artemisinin in Artemisia annua L. through Induced Mutation. Open Access Library Journal, 2, e2189. doi: http://dx.doi.org/10.4236/oalib.1102189.
Simon, J.E., Chadwick, A.F. and Craker, L.E. (1984) Herbs: An Indexed Bibliography, 1971-1980. The Scientific
Literature on Selected Herbs, and Aromatic and Medicinal Plants of the Temperate
Zone. Archon Books, Hamden.
Luo, X.D. and Shen,
C.C. (1987) The
Chemistry, Pharmacology, and Clinical Applications of Artemisinin and Its Derivatives. Medicinal Research Reviews, 7, 29-52. http://dx.doi.org/10.1002/med.2610070103
Ogwang, P.E., Ogwal, J.O., Kasasa, S., Olila,
D., Ejobi, F., Kabasa, D. and Obua, C.
(2012) Artemisia annuaL. Infusion Consumed Once a Week Reduces Risk of
Multiple Episodes of Malaria: A Randomized Trial in a Ugandan Com- munity. Tropical Journal of Pharmaceutical
Research, 13,
445-453.
Van Agtmael, M.A., Eggelte, T.A.
and van Boxtel, C.J. (1999) Artemisinin Drugs in the Treatment of Malaria: From
Medicinal Herb to Registered Medication. Trends
in Pharmacological Sciences, 20, 199-205. http://dx.doi.org/10.1016/S0165-6147(99)01302-4
Willcox, M.L., Burton, S., Oyweka, R.,
Namyalo, R., Challand, S. and Lindsey, K. (2011) Evaluation and Pharmacovigilance of Projects
Promoting Cultivation and Local Use of Artemisia
annua for Malaria. Malaria Journal, 10, 84. http://dx.doi.org/10.1186/1475-2875-10-84
Khan, S. and Al-Qurainy, F. (2010) Mutagenic Effect of Sodium Azide on Seed
Germination of Eruca sativa (L.) Aust. Journal of Basic and
Applied Sciences, 3, 3081-3087.
Weathers, P.J. and Towler, M.J. (2012) The Flavonoids Castic in and Artemetin
Are Poorly Extracted and Are Unstable in an Artemisia
annua Tea Infusion. Planta Medica, 78,
1024-1026. http://dx.doi.org/10.1055/s-0032-1314949
Jaetzold, R. and Schimdt, H. (1983) Farm Management Handbook of Kenya.
Vol. II. Natura Condition
and Farm Management Information. Ministry of Agriculture, Kenya in Cooperation
with German Agriculture Team (GAT) of the German Agency for Technical
Cooperation (GTZ) Nairobi Kenya.
Sreevidya, T.V. and Narayama, B. (2007) Spectrophotometric
Determination of Artemisinin and Dihydroartemisinin. Indian Journal of Chemical Technology, 15, 59-62.
Delabays, N., Benakis, A. and Collet, G. (1993) Selection and Breeding
for High Artemisinin Yielding Strains of Artemisia
annua. Acta Horticulturae, 330, 203-206. http://dx.doi.org/10.17660/ActaHortic.1993.330.24
Koobkokkruad, T., Chochai, A., Kirdmanee, C. and De-Eknamkul, W. (2008) Effects of Low-Dose Gamma Irradiation
Omartemesinin Content and Amorpha-4, 11-Diene Synthase Activity in Artemisia annua L. International Journal of Radiation Biology, 84,
878-884. http://dx.doi.org/10.1080/09553000802460131
Khan, M.R.,
Qureshi, A.S.,
Hussain, S.A. and Ibrahim, M. (2005) Genetic
Variability Induced by Gamma Irradiation and Its Modulation with Gibberelline
Acid in M2 Generation of Chickpea (Cicer arietinum L.). Pakistan Journal of Botany, 37,
285-292.
Singh, M. (2001) Effect of Nitrogen,
Phosphorus and Potassium Nutrition on Herb, Oil and Artemisinin Yield of Artemisia annua under Semi-Arid Tropical
Condition. Joint Milli-Arcsecond
Pathfinder Survey, 23,
368-369.
Barbacka, K. and Baer-Dubowska, W. (2011) Searching for Artemisinin Production
Improvement in Plants and Microorganisms. Current
Pharmaceutical Biotechnology, 12, 1743-1751. http://dx.doi.org/10.2174/138920111798376923
Chalapathi, M.V., Vasundhara, M., Gangadhar Eswar Rao, G., Thimmegowda, S., Rao Prakash, E.V.S. and Devakumar,
N. (2004) Influence of Integrated Nutrient Management on Yield of Davana (Artemisia pallens). Indian Perfumer, 48, 311-316.
Liu, C.-Z.,
Guo,
C., Wang, Y.
and Ouyang, F. (2003) Factors Influencing Artemisinin Production from Shoot
Cultures of Artemisia annua L. World Journal of Microbiology and Biotechnology, 19,
535-538. http://dx.doi.org/10.1023/A:1025158416832
Duke, S.O. and Paul, R.N. (1993) Development
and Fine Structure of Glandular Trichomes of Artemisia annuaL. International
Journal of Plant Sciences, 154, 107-118. http://dx.doi.org/10.1086/297096
Mergen, F. and Johnson, T.S. (1964) Effect of Ionizing Radiation in Seed
Germination and Seedling Growth of Pinusrigida (MU). Radiation Botany, 4,
417-427. http://dx.doi.org/10.1016/S0033-7560(64)80009-0
Farhi, M., Kozin, M.,
Duchin, S. and Vainstein, A. (2013) Metabolic Engineering of Plants for
Artemisinin Synthesis. Biotechnology and
Genetic Engineering Reviews, 29, 135-148. http://dx.doi.org/10.1080/02648725.2013.821283
Brown, G.D.
(2010) The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia
annuaL. (Qinghao). Molecule, 15, 7603-7698. http://dx.doi.org/10.3390/molecules15117603
Soetaert, S.S.A.,
Van Neste, C.M.F.,
Vandewoestyne, M.L.,
Head, S.R.,
Goossens, A.,
Van Nieuwerburgh, F.C.W. and Deforce, D.L.D. (2013) Differential Transcriptome Analysis of
Glandular and Filamentous Trichomes in Artemisia annua. BMC Plant Biology, 13, 220. http://dx.doi.org/10.1186/1471-2229-13-220
Laughlin, J.C. (1994) Agricultural Production of Artemisinin—A
Review. Transactions of
the
Royal Society of Tropical Medicine and Hygiene, 88, 21-22. http://dx.doi.org/10.1016/0035-9203(94)90465-0
Sushil,
K., Gupta, S.K., Poorinima, S., Pratima, B., Gupta, M.M., Digvijay, S., Gupta,
A.K., Govind, R., Shasany, A.K. and Srikant, S. (2004) High Yields of Artemisinin by Multi-Harvest of Artemisia annuaCrops. Industrial Crops and
Products, 19, 77-90.