Bmp and activin membrane-bound inhibitor (BAMBI) was initially described as
a pseudo receptor antagonizing TGF-β receptor
activation, thus impairing signaling. This study tries to find the expression and
its significance of BAMBI in breast cancer and explore the relation between BAMBI
and clinical and pathological factors of breast cancer. The expression of BAMBI
was studied using three cell lines model and Immunofluorescence staining. The impact of BAMBI expression on breast cell was estimated by Western
blot and Semi-quantitative RT-PCR. It was demonstrated that BAMBI protein expressed
mainly in the membrane and the cytoplasm close to the membrane in breast cancer
cell lines, and the relationship between the expression of BAMBI and the proliferation
and migration of breast cancer for the first time. The order of BAMBI mRNA expression
in the three breast cancer lines is MDA-MB-435s > MDA-MB-231 > MCF-7. Respectively
the difference was significant (P <
0.05). It agrees with the proliferation and migration of the three cell lines. The
relative amount of BAMBI protein in MDA-MB-435s, MDA-MB-231 and MCF-7 was 0.963
± 0.061, 0.957 ± 0.048, 0.769 ± 0.103, and the difference was significant (P <0.05). The abnormally up- regulation of BAMBI is related to breast
carcinogenesis.
Cite this paper
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