Effect of Morphological and Physiological Development on the Acclimatization of in Vitro Plants of Bambusa vulgaris Schrad ex Wendl in Liquid Culture Medium
Bambusa
vulgaris Schrad. ex Wendl (B. vulgaris)
is a species of great interest because it produces the most important
non-timber forest products of worldwide. It has become an integral component
in social forestry programs. This study aimed to determine the effect of
morphological and physiological development on the acclimatization of in vitro plants of B. vulgaris. Morphological and physiological parameters of plants
subjected to in vitro conditions were
evaluated. Our results showed that the number shoots per explant of B. vulgaris increased when 6.0 μM of
6-benzylade- nine (BA) was incorporated into the culture medium. After six
subcultures, clumps of 3 - 6 axes were obtained, and their division in groups
of three axes allowed multiplication of the plants. During the acclimatization
phase to ex vitro conditions, the in vitro plants showed 75% survival.
Results showed that greater development of the root system, height and number
of leaves per plants was observed with height larger of 3.0 cm. The leaf area,
water content, fresh and dry weight
increased with height larger of 3.0 cm, but all parameters were decreased with
height (1.0 - 3.0 cm). The correlation between net assimilation rate and leaf
area index was significantly positive in
vitro plants with height larger of 3.0 cm. The increase of plants of B. vulgaris in liquid culture medium
offers new prospects for increasing the commercial production of this species,
and for studies that could elucidate morphological and physiological responses
in plants of bamboo.
Cite this paper
Garcia-Ramirez, Y. , Gonzá, M. , lez-Gonzá, lez, Freire-Seijo, M. , O-Cá, M. L. , rdenas, Leó, M. , n-Quintana, Roque-Morales, B. , Mena-Mé, E. , ndez, Mendoza-Rodriguez, M. F. and Rivero-Quintana, L. (2015). Effect of Morphological and Physiological Development on the Acclimatization of in Vitro Plants of Bambusa vulgaris Schrad ex Wendl in Liquid Culture Medium. Open Access Library Journal, 2, e1787. doi: http://dx.doi.org/10.4236/oalib.1101787.
Ramanayake, S.,
Meemaduma, V. and Weerawardene, T. (2006) In Vitro Shoot Proliferation and Enhancement of Rooting for the Large-Scale Propagation of Yellow Bamboo (Bambusa vulgaris “Striata”). Scientia Horticulturae, 110, 109-113. http://dx.doi.org/10.1016/j.scienta.2006.06.016
Ramanayake, S., Maddegoda, K.,
Vitharana, M. and Chaturani, G. (2008) Root Induction in Three Species of Bamboo with
Different Rooting Abilities. Scientia
Horticulturae, 118, 270-273. http://dx.doi.org/10.1016/j.scienta.2008.06.004
Aitken-Christie, J., Kozai, T. and Takayama, S. (1995) Automation in Plant Tissue Culture—General
Introduction and Overview. In: Aitken-Christie, J., Kozai, T. and Smith, L., Eds., Automation and Environmental
Control in Plant Tissue Culture, Kluwer Academic Publishers, Dordrecht, 1-18.
Mudoi, K.D., Saikia, S.P., Goswami, A.,
Gogoi, A., Bora, D. and Borthakur, M. (2013) Micropropagation of Important Bamboos: A Review. African Journal of Biotechnology, 12,
2770-2785.
Bisht, P., Pant, M. and Kant, A. (2010) In Vitro Propagation of Gigantochloa atroviolaceae Widjaja through Nodal Explants. Journal of American Science, 6, 1019-1026.
Pratibha,
S. and Sarma, K.P. (2013) In Vitro Propagation of Bambusa tulda: An Important
Plant for Better Environment. Journal of Environmental Research
and Development, 7, 1216-1223.
Jiménez, V., Castillo, J., Tavares, E., Guevara, E. and Montiel, M. (2006) In Vitro Propagation of the Neotropical
Giant Bamboo, Guadua angustifolia Kunth, through Axillary Shoot Proliferation. Plant Cell, Tissue and Organ
Culture, 86, 389-395. http://dx.doi.org/10.1007/s11240-006-9120-4
Ndiaye, A., Mamadou, S.,
Niang, D. and Gassama-Dia, Y. (2006) In Vitro Regeneration of Adult Trees of Bambusa
vulgaris. African Journal of Biotechnology, 5, 1245-1248.
Sayanika,
W. and Sharma, G. (2009) In Vitro Propagation of Arundinaria callosa Munro—An Edible Bamboo from Nodal Explants of Mature Plants. The Open Plant Science Journal, 3, 35-39. http://dx.doi.org/10.2174/1874294700903010035
Aragón, C.,
Carvalho, L., González, J., Escalona, M. and Amancio, S. (2010) Ex Vitro Acclimatization of Plantain
Plantlets Micropropagated in Temporary Immersion Bioreactor. Biologia Plantarum, 54, 237-244. http://dx.doi.org/10.1007/s10535-010-0042-y
Cha-um, S., Puthea, O. and
Kirdmanee, C. (2009) An Effective In-Vitro Acclimatization Using Uniconazole Treatments and Ex-Vitro Adaptation of Phalaenopsis orchid. Scientia Horticulturae,
121, 468-473. http://dx.doi.org/10.1016/j.scienta.2009.02.027
Quiala, E., Cannal, M.,
Meijón, M., Rodriguez, R., Chávez, M., Valledor, L., de Feria, M. and Barbón, R. (2012) Morphological
and Physiological Responses of Proliferating Shoots of Teak to Temporary
Immersion and BA Treatments. Plant Cell, Tissue and Organ
Culture (PCTOC), 109, 223-234. http://dx.doi.org/10.1007/s11240-011-0088-3
Hurtado,
O., Freire-Seijo, M., Leiva-Mora, M. and García-Ramírez, Y. (2011) Influencia de las características de las
plantas cultivadas in Vitro de Bambusa vulgaris var. vulgaris
Schrad. ex Wendl. en
su aclimatización. Biotecnología Vegetal, 11, 155-161.
Haworth, M., Elliott-Kingston, C. and McElwain, J.C. (2013) Co-Ordination of Physiological and Morphological
Responses of Stomata to Elevated [CO2] in Vascular Plants. Oecologia,
171, 71-82. http://dx.doi.org/10.1007/s00442-012-2406-9