Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker
Rhizophora mangle L. is a vegetal
species with high disponibility in Cuba for guarantying
economical factibility by drug development.
Preview studies showed promissory pharmacological results by the use of this
plant in the treatment of gastroduodenal ulcers. The present work involved another
evaluation of the active extract important by the development of a new drug. It
was evaluated by intestinal absorption in vitro and in situ models
and anti-diarrheic activity in rats. The polyphenolic fractions were separated
from the aqueous extract and its cytoprotective activity was studied on gastric
ulceration induced by ethanol plus hydrochloric acid in rats. Some toxicological
studies were carried out. We made a validation of HPLC method by the analysis
of one mark in the tablet development. The aqueous extract showed high
absorption, and high anti-diarrheic effect. The low-molecular weight
polyphenolic fraction showed the highest level of gastric protection. No
toxicological signs were obtained by it. HPLC method evidenced linearity, precision, sensitivity and accuracy.
Cite this paper
Perera, L. M. S. , Piloto, J. , Canelsota, D. , Pelzer, L. and Mancebo, B. (2016). Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker. Open Access Library Journal, 3, e1625. doi: http://dx.doi.org/10.4236/oalib.1101625.
Sánchez, P.L.M., Niurka, Y.B., Rodríguez, A., Farrada, F. and Bulnes, C. (2004) Gastric and Duodenal Antiulcer Effects of Rhizophora mangle L.Pharmaceutical Biology, 42, 225-229. http://dx.doi.org/10.1080/13880200490514096
Berenguer, B., Sánchez, L.M., Quilez, A., López-Barreiro, M., de Haro, O., Gálvez, J. and Martin, M.J.J. (2006) Protective and Antioxidant Effects of Rhizophora mangle L. against NSAID-Induced Gastric Ulcers. Journal of Ethnopharmacology, 103, 194-200. http://dx.doi.org/10.1016/j.jep.2005.08.029
Sánchez, P.L.M., Melchor, G., álvarez, S. and Bulnes, C. (1998) Chemical and Toxicological Characterization of One Wound Healing Formulation
form Rhizophora mangle L. Revista de Salud Animal, 20, 69-72.
Edith, L., Steingas, H. and Menice, K.H. (1991) Tannins in Ruminant
Feed Stuffs. Separate Print Animal by the Institute for Scientific
Cooperation. Institute
for Scientific Cooperation, 33, 9-62.
Sánchez, P.L.M., Varcalcel, L., Escobar, A. and Noa, M. (2008b) Polyphenol and Phytosterols. Composition in an
Antibacterial Extract from RhizophoramangleL.’s Bark. Journal of Herbal
Pharmacotherapy, 7, 107-128. http://dx.doi.org/10.1080/15228940802142720
Labieniec, M. and Gabryelak, T. (2003) Effects of Tannins on Chinese Hamster
Cell Line B14. Mutation Research, 39, 127-135. http://dx.doi.org/10.1016/S1383-5718(03)00161-X
Pino, J.A., Marbot, R., Aguero, J. and Sánchez, L.M. (2001) Volatile Components of Red Mangrove Bark (Rhizophora mangle L.) from Cuba. Journal of Essential Oil Research, 13, 88-89. http://dx.doi.org/10.1080/10412905.2001.9699622
Sánchez, P.L.M., Ivis, F.C., Betty, M.D. and Rafael, L.M. (2008a) Oral Acute and Subacute Toxicological of Freeze Dried Aqueous Extract of Rhizophoramangle L. in Rats. Revista
Cubana de Plantas Medicinales, 13, 1-13.
Travieso, M.C., Hechavarria, O., Betancourt, A., Frontela, M. and Miranda, I. (1998) Validación del método de
determinación de taninos totales en productos naturales vegetales. Revista
de Protección Vegetal, 13,
189-194.
González
álvarez, M.A.
(2002) Estudio in situ e in vitro de
los mecanismos de absorción y secreción intestinal del 3’metilciprofloxacino.
Doctoral Thesis Doctoral,
Facultad de Farmacia, Universidad
de Valencia, Valencia.
Wyrobek, A.J. and Bruce, W.R. (1978) The Induction of Sperm Shape Abnormalities in Mice and
Humans. Chemical
Mutagens: Principles and Methods for Their Detection. A Hollander and FH de
Serres Plenum Press, New
York, 257- 285.
Gajewski, D.M. (2000) A Induction of Micronuclei in Bone Marrow and Sperm Head
Abnormalities after Combined Exposure of Mice to Low Doses of X-Rays and
Acrylamide. Teratogenesis Carcinogenesis
Mutagenesis Journal, 20, 133-140.
Klaude, M., Ericson, S., Nygren, J. and Ahnstro, G. (1996) The Comet Assay: Mechanisms and Technical Considerations. Mutation Research, 363, 89-96. http://dx.doi.org/10.1016/0921-8777(95)00063-1
Schapoval, E.E.S., Silveira, S.M., Miranda, M.L., Alice, C.B. and Henriques, A.T. (1994) Evaluation of Some Pharmacological Activities of EugeniaunifloraL. Journal of
Ethnopharmacology, 44, 137-142. http://dx.doi.org/10.1016/0378-8741(94)01178-8
Konig, M., Scholz, E., Hartmann, R., Lehmann, W. and Rimpler, H. (1994) Ellagitannins and
Complex Tannins from QuercuspetraeaBark. Journal of Natural Products, 57, 1411-1415. http://dx.doi.org/10.1021/np50112a010
Duke, J.A. (2005) Chemicals and Their
Biological Activities in: Capsicum Annuum L. Dr. Duke’s Phytochemical and Ethnobotanical Databases. USDA-ARS-NGRL, Beltsville
Agricultural Research Center, Beltsville. http://www.ars-grin.gov/cgi-bin/duke/farmacy2.pl
Ramírez, R.O. and Roa, C.C. (2003) The Gastroprotective Effect of Tanninsextracted from Duhat
(Syzygiumcumini Skeels)
Bark on HCl/Etanolinduced Gastric Mucosal Injury in Sprague-Dawley Rats. Clinical Hemorheology and Microcirculation, 29, 253-261.
Stehagen, E.,
Abrahamsan, S. and McLafferty, F.W. (1974) Registry of Mass Spectral Data, Vol. 1. A Wiley Interscience
Publication, John Wiley & Sons, New York, London, 1-826.
Haslam, E. (1996)
Natural Polyphenols (Vegetable Tannins) as Drugs: Possible
Modes of Action. Journal of Natural Products, 59, 205-215. http://dx.doi.org/10.1021/np960040
Fernández, O., Capdevila, J.Z., Dalla, G. and Melchor, G. (2002) Efficacy of Rhizophora mangleAqueous Bark Extract in the Healing of Open Surgical Wounds. Fitoterapia, 73, 564-568. http://dx.doi.org/10.1016/S0367-326X(02)00229-0
Aguwa, C.N. and Nwako, S.O. (1988) Preliminary Studies of the Root Extracts of NauclealatifoliaSmith, for Anti- Ulcer Properties. Nigerian
Journal of Pharmaceutical Sciences, 4, 16-23.
Nwafor, P.A., Effraim, K.D. and Jacks, T.W. (1996) Gastroprotective Effects of Aqueous
Extract of KhayasenegalensisBark on Indomethacin Induced Ulceration in Rats. West African Journal of Pharmacology and
Drug Research, 12, 46-50.
Nwafor, P.A., Okwuasaba, F.K. and Binda, L.G. (2000) Antidiarrhoeal and Antiulcerogenic Effects of Methanolic
Extract of Asparagus pubescensroot in Rats. Journal of Ethnopharmacology, 72, 421-427. http://dx.doi.org/10.1016/S0378-8741(00)00261-0
Al-Rehaily, A.J.,
Al-Howiriny, T.A., Al-Sohaibani, M.O. and Rafatullah, S. (2002) Gastroprotective Effects of “Amla” Emblicaofficinalison in Vivo Test Models in Rats. Phytomedicine, 9, 515-522. http://dx.doi.org/10.1078/09447110260573146
Tsuyama, Y., Uchida, T. and Goto, T.J. (1992) Analysis of Underivatized C12 to C18 Fatty
Acids by Reversed Phase Ion-Pair-High Performance Liquid
Chromatography with Conductivity Detection. Journal of Chromatography, 596, 181-184. http://dx.doi.org/10.1016/0021-9673(92)85005-E
Jayaraj, A.P., Tovey, F.I. and Hobsley, M. (2003) Duodenal Ulcer Prevalence: Research
into the Nature of Possible Protective Dietary Lipids. Phytotherapy Research, 17, 391-398. http://dx.doi.org/10.1002/ptr.1310
Hobsley, M. and Tovey, F.I. (2001) Helicobacter pylori: The Primary Cause of Duodenal
Ulceration or a Secondary Infection? World
Journal of Gastroenterology, 7, 149-150.
Meshkatal-Sadat, M.H. and Parekh, H.H. (1990) Chemical Investigations of the Flowers of Moltkiacaerulea. PlantaMedica, 56, 556 -557. http://dx.doi.org/10.1055/s-2006-961140
Cancino, L., Leiva, A., Garrido, G., Cossio, A. and Prieto, E. (2003) Vimang:
Los efectos antigenotóxico y modulador de las enzimas glutatión peroxidasa y
glutatión-S-transferasa. Revista Cubana
de Investigaciones Biomédicas, 20, 48- 53.
Remigio, A. (2002) Evaluación genotóxica de seis
extractos de plantas medicinales, mediante ensayos in vivo en ratones [Tesis de Maestría]. Facultad de Farmacia y
Alimentos, Universidad de La Habana, Ciudad de La Habana.
Wyrobek, A. and Bruce, W.
(1975) Chemical Induction of Sperm Abnormalities in Mice. Proceedings
of the National Academy of Sciences of the United States of America, 72, 4425-4429. http://dx.doi.org/10.1073/pnas.72.11.4425
González, E., Iglesias, I., Carretero, E. and Villar, A. (2000) Gastric Cytoprotection of Bolivian Medicinal Plants. Journal of Ethnopharmacology, 70, 329-333. http://dx.doi.org/10.1016/S0378-8741(99)00183-X
Nair, C.K.K. and Salvi, V.P. (2008) Protection of DNA from Gamma-Radiation Induced Strand
Breaks by Epicatechin. Mutation Research, 650, 48-54. http://dx.doi.org/10.1016/j.mrgentox.2007.10.001
Carbonaro, M., Grant, G. and Pusztai, A. (2001) Evaluation of Polyphenol Bioavailability in Rat Small
Intestine. European Journal of Nutrition, 56, 269-316. http://dx.doi.org/10.1007/s003940170020
Gee, J.M. and Jonson, I.T. (2001)
Polyphenolic Compunds: Interactions with the Gut and Implications for Human
Health. Current Medicinal Chemistry, 56, 269-316.
Manach, C.,
Scalbert, A., Morand, C. and Jiménez, L. (2004) Polyphenols: Food Sources and Bioavailability. American Journal of Clinical Nutrition, 56, 269-316.
Spencer, J.P., Schroeter, H.,
Crossthwaithe, A.J., Kuhnle, G., Williams, R.J. and Rice-Evans, C. (2001) Contrasting Influences of Glucuronidation and O-Methylation
of Epicatechin on Hydrogen Peroxide-Induced Cell Death in Neurons and Fibroblasts. Free Radical Biology & Medicine, 31, 1139-1146. http://dx.doi.org/10.1016/S0891-5849(01)00704-3
Scalbert, A., Déprez, S., Mila, I., Albrecht, A.M., Huneau, J.F. and
Rabot, S. (2000) Proanthocyanidins and Human Health: Systematic Effects
and Local Effects in the Gut. Biofactors, 13, 115-120.
Ezaki, N., Kato, M., Takizawa, N., Morimoto, S., Nonaka, G.I., and Nishioka, I. (1985) Pharmacological Studies
on LinderaeumbellataeRamus. IV. Effects of Condensed Tannin
Related Compounds on Peptic Activity and Stress-In- duced Gastric Lesions in Mice. Planta
Medica,52, 34-38. http://dx.doi.org/10.1055/s-2007-969386
Hung, C.R. and Lee, C.H. (1991) Protective Effect
of Cimetidine on Tannic Acid—Induced Gastric Damage in Rats. Journal
of Pharmacy and Pharmacology, 56, 269-316. http://dx.doi.org/10.1111/j.2042-7158.1991.tb03536.x
Tebid, K., Besancon, P. and Rovanet, J.M. (1996) Effects of Dietary Grape Seed Tannins on Rat Cecal
Fermentation and Colonic Bacterial Enzymes. Nutrition Research, 16, 105-110. http://dx.doi.org/10.1016/0271-5317(95)02064-0