As more and more genetically modified (GM) crops are approved for commercialization and planting, safety issues of GM crops have become hot topics worldwide. For both regulatory and academic purposes, development of rapid, economic and effective on-site detection methods for GM components is indispensible. Up to now, the most effective and sensitive techniques used for GMO detection are based on polymerase chain reaction (PCR). PCR method needs expensive, heavy instruments and gel electrophoresis, therefore, it is commonly used in laboratory test, and unsuitable for on-site detection. Loop-mediated isothermal amplification (LAMP), an isothermal nucleic acid amplification technique, has been extensively used in many areas such as food safety and clinic diagnosis. Advantageous characteristics of LAMP, such as high specificity and sensitivity, simple operation, low cost, eye visualization, particularly free of special equipment, renders it with high potential to be used for GMO on-site detection. In this review, we summarized current status of the application of LAMP in GMO detection, and discussed possible improvements needed for its adaptability regarding to on-site GMO detection. Hopefully, the information present here would facilitate the practical risk assessment of GMO.
Cite this paper
Li, R. , Wang, C. , Ji, L. , Zhao, X. , Liu, M. , Zhang, D. and Shi, J. (2015). Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives. Open Access Library Journal, 2, e1264. doi: http://dx.doi.org/10.4236/oalib.1101264.
Smythe, S.,
Kerr, W.A. and Davey, K.A. (2006) Closing
Markets to Biotechnology: Does It Pose an Economic Risk If Markets Are Globalised? International Journal of Technology and
Globalisation, 2, 377-389. http://dx.doi.org/10.1504/IJTG.2006.011923
Broeders, S.R.M.,
Keersmaecker, S.C.J. and Roosens, N.H.C. (2012) How
to Deal with the Upcoming Challenges in GMO Detection in Food and Feed. BioMed Research International, 2012, Article ID: 402418.
Bonfini, L. (2002) Review of GMO Detection and Quantification
Techniques. Institute for Health and Consumer Protection. Food Products and
Consumer Goods Unit, 1-74.
Zhang, D.B.
and Guo, J.C. (2011) The Development and Standardization of Testing
Methods for Genetically Modified Organisms and Their Derived Products. Journal of Integrative Plant Biology, 53,
539-551. http://dx.doi.org/10.1111/j.1744-7909.2011.01060.x
Randhawa, G.J.,
Singh, M. and Morisset, D. (2013) Loop-Mediated Isothermal Amplification: Rapid Visual
and Real- Time
Methods for Detection of Genetically Modified Crops. Journal of Agricultural and Food Chemistry, 61, 11338- 11346. http://dx.doi.org/10.1021/jf4030085
James, C.
(2013) Global
Status of Commercialized Biotech/GM Crops: 2013; ISAAA Brief No. 46.
International Service for the Acquisition Agri-Biotech Applications (ISAAA),
Ithaca.
Gruere, G.P.,
Carter, C.A. and Hossein, F.
(2009) Explaining International Differences in Genetically
Modified Food Labeling Policies. Review of International Economics, 17, 393-408. http://dx.doi.org/10.1111/j.1467-9396.2008.00788.x
Marmiroli, N.,
Maestri, E., Gullì, M., Malcevschi, A., Peano, C., Bordoni, R. and De
Bellis, G. (2008) Methods for
Detection of GMOs in Food and Feed. Analytical
and Bioanalytical Chemistry, 392, 369-384. http://dx.doi.org/10.1007/s00216-008-2303-6
Arne, H.J.,
Marc, D.L. and Guy, V.D.E. (2006) Coherence between Legal Requirements and Approaches for Detection of
Genetically Modified Organisms (GMOs) and Their Derived Products. Journal of Agricultural and Food Chemistry,
54, 2799-2809. http://dx.doi.org/10.1021/jf052849a
Mori, Y. and Notomi, T. (2009) Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Accurate, and
Cost- Effective Diagnostic Method for Infectious
Diseases. Journal of Infection and
Chemotherapy, 15, 62-69. http://dx.doi.org/10.1007/s10156-009-0669-9
Mori, Y., Kanda, H.
and Notomi, T. (2013) Loop-Mediated Isothermal Amplification (LAMP):
Recent Progress in Research and Development. Journal of Infection and Chemotherapy, 19, 404-411. http://dx.doi.org/10.1007/s10156-013-0590-0
Francois, P.,
Tangomo, M.,
Hibbs, J.,
Bonetti, E.J.,
Boehme, C.C., Notomi, T.,
Perkins, M.D. and Schrenzel, J. (2011) Robustness of a Loop Mediated Isothermal
Amplification Reaction for Diagnostic Applications. FEMS Immunology and Medical Microbiology, 62, 41-48. http://dx.doi.org/10.1111/j.1574-695X.2011.00785.x
Kiddle, G.,
Hardinge, P.,
Buttigieg, N.,
Gandelman, O.,
Pereira, C.,
McElgunn, C.,
Rizzoli, M.,
Jackson, R., Appleton, N.,
Moore, C.,
Tisi, L. and Murray, J. (2012) GMO Detection Using a Bioluminescent Real Time Reporter (BART) of Loop
Mediated Isothermal Amplification (LAMP) Suitable for Field Use. BMC Biotechnology, 12, 15. http://dx.doi.org/10.1186/1472-6750-12-15
Asiello, P.J. and Baeumner, A.J.
(2011) Miniaturized Isothermal Nucleic Acid Amplification: A
Review. Lab on a Chip, 11,
1420-1430. http://dx.doi.org/10.1039/c0lc00666a
Fukuta, S.,
Mizukami, Y.,
Ishida, A.,
Ueda, J.,
Hasegawa, M.,
Hayashi, I.,
Hashimoto, M. and Kanbe, M. (2004) Real- Time
Loop-Mediated Isothermal Amplification for the CaMV-35S Promoter as a Screening
Method for Genetically Modified Organisms. European
Food Research and Technology, 218, 496-500. http://dx.doi.org/10.1007/s00217-003-0862-5
Lee, D.,
La, M.M.,
Allnutt, T. and Powell, W. (2009) Detection of Genetically Modified Organisms (GMOs) Using Isothermal
Amplification of Target DNA Sequences. BMC
Biotechnology, 9, 7. http://dx.doi.org/10.1186/1472-6750-9-7
Chen, J.,
Huang, C.,
Zhang, X.,
Yu, R. and Wu, Z. (2011) Detection of Herbicide-Resistant Maize by Using Loop- Mediated Isothermal
Amplification of the Pat Selectable Marker Gene. African Journal of Biotechnology, 10,
17055- 17061.
Li, Q.,
Fang, J.,
Liu, X.,
Xi, X.,
Li, M.,
Gong, Y. and Zhang, M. (2013) Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid
Detection of cry1Ab Gene in Transgenic Rice (Oryza
sativa L.). European Food Research and Technology, 236, 589-598. http://dx.doi.org/10.1007/s00217-013-1911-3
Li, F.W.,
Yan, W.,
Long, L.K.,
Qi, X.,
Li, C.C. and Zhang, S.H. (2014) Development and Application of Loop- Mediated Isothermal Amplification Assays for Rapid Visual Detection of cry2Ab and cry3A Genes in Genetically- Modified Crops. International Journal of Molecular Sciences, 15, 15109-15121. http://dx.doi.org/10.3390/ijms150915109
Chen, L.L.,
Guo, J.C.,
Wang, Q.D.,
Kai, G.Y. and Yang, L.T. (2011) Development of the Visual LAMP Assays for Seven Genetically Modified
Maize Events and Their Application in Practical Samples Analysis. Journal of Agricultural and Food Chemistry,
59, 5914-5918. http://dx.doi.org/10.1021/jf200459s
Niloofar, R.,
Ali, H.,
Masoud, T. and Aboubakr, M. (2011) Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by
Loop-Mediated Isothermal Amplification. Czech
Journal of Genetics and Plant Breeding, 47, 140- 148.
Zhang, M.,
Liu, Y.N.,
Chen, L.L.,
Quan, S.,
Jiang, S.M.,
Zhang, D.B. and Yang, L.T. (2013) One Simple DNA Extraction Device and Its Combination with Modified
Visual Loop-Mediated Isothermal Amplification for Rapid On-Field Detection of
Genetically Modified Organisms. Analytical
Chemistry, 85, 75-82. http://dx.doi.org/10.1021/ac301640p