The purified Immunoglobulin G (IgG) is effectively used in passive
immunization. There are various methods to isolate the IgG from serum, with its
own advantages and disadvantages. In the current study, a comparative efficacy
of the affinity chromatography using Protein A—Sepharose and ion
exchange chromatography using DEAE Sephadex A50 was done with regard to yield
and purity of IgG. Both methods were found equally effective in isolation of
pure IgG with similar recovery, indicating that researcher can use either
method to purify IgG depending on available resources.
Cite this paper
Rajput, M. , Mansour, S. M. G. , Braun, L. J. , Darweesh, M. , Thakur, N. and Chase, C. C. L. (2014). Comparison of the Affinity Chromatography and the Ion Exchange Chromatography in the Isolation of Bovine Immunoglobin G. Open Access Library Journal, 1, e960. doi: http://dx.doi.org/10.4236/oalib.1100960.
Haas, L.F. (2001) Emil Adolph von Behring (1854-1917) and Shibasaburo Kitasato (1852-1931). Journal of Neurology, Neurosurgery and Psychiatry, 71, 62.
http://dx.doi.org/10.1136/jnnp.71.1.62
Schroeder Jr., H.W. and Cavacini, L. (2010) Structure and Function of Immunoglobulins. The Journal of Allergy and Clinical Immunology, 125, 41-52. http://dx.doi.org/10.1016/j.jaci.2009.09.046
Hvatum, M., Scott, H. and Brandtzaeg, P. (1992) Serum IgG Subclass Antibodies to a Variety of Food Antigens in Patients with Coeliac Disease. Gut, 33, 632-638. http://dx.doi.org/10.1136/gut.33.5.632
Mallery, D.L., McEwan, W.A., Bidgood, S.R., Towers, G.J., Johnson, C.M. and James, L.C. (2010) Antibodies Mediate Intracellular Immunity through Tripartite Motif-Containing 21 (TRIM21). Pro-ceedings of the National Academy of Sciences of the United States of America, 107, 19985-19990. http://dx.doi.org/10.1073/pnas.1014074107
Bush, L.J. and Staley, T.E. (1980) Absorption of Colostral Immunoglobulins in Newborn Calves. Journal of Dairy Science, 63, 672-680. http://dx.doi.org/10.3168/jds.S0022-0302(80)82989-4
Gapper, L.W., Copestake, D.E., Otter, D.E. and Indyk, H.E. (2007) Analysis of Bovine Immunoglobulin G in Milk, Colostrum and Dietary Supplements: A Review. Analytical and Bioanalytical Chemistry, 389, 93-109. http://dx.doi.org/10.1007/s00216-007-1391-z
Guarino, A., Canani, R.B., Russo, S., Albano, F., Canani, M.B., Ruggeri, F.M., Donelli, G. and Rubino, A. (1994) Oral Immunoglobulins for Treatment of Acute Rotaviral Gastroenteritis. Pediatrics, 93, 12-16.
Kiraly, J., Vareckova, E., Mucha, V. and Kostolansky, F. (2011) Evaluation of Anti-Influenza Efficiency of Polyclonal IgG Antibodies Specific to the Ectodomain of M2 Protein of Influenza A Virus by Passive Immunization of Mice. Acta Virologica, 55, 261-265. http://dx.doi.org/10.4149/av_2011_03_261
Pare, J., Hietala, S.K. and Thurmond, M.C. (1995) An Enzyme-Linked Immunosorbent Assay (ELISA) for Serological Diagnosis of Neospora sp. Infection in Cattle. Journal of Veterinary Diagnostic Investigation, 7, 352-359. http://dx.doi.org/10.1177/104063879500700310
