This study was performed to evaluate the antibiotic
sensitivity pattern and determination of MIC (Minimum inhibitory concentration)
and MBC (Minimum bactericidal concentration) value of Pseudomonas aeruginosa. The samples were collected from
hospitalized and outdoor patients of Chittagong Maa-O-Shisu General hospital (October,
2010 to April, 2011). From the sample (blood, pus and urine) the isolate was
identified on the basis of their morphological, cultural and biochemical
characteristics. Antibiotic sensitivity pattern of the isolates was then
observed (Disc diffusion method). It was found that Ciprofloxacin, Azithromycin
and Chloramphenicol showed the highest percentages of sensitivity (above 60%)
so they were the most effective drug for the treatment of Pseudomonas aeruginosa infection,
where Vancomycin, Amphicillin and Amoxicillin showed the lowest
percentages of sensitivity as well as less effective drug. Minimum Inhibitory
Concentration (MIC) (Turbidimetric Method) values were determined against six
antibiotics (Ciprofloxacin, Azithromycin, Kanamycin, Erythromycin,
Chloramphenicol and Gentamycin). The MIC of Ciprofloxacin and Azithromycin for
the majority of clinical isolates was 2 to >64 μg/ml. Like that the MIC of
Gentamycin, Kanamycin and Chloramphenicol for the clinical isolates was 4 to
>64 μg/ml. MIC value of Erythromycin was between 8 to >64 μg. The Minimum
bactericidal concentration (MBC) for all these six drugs were also done and the
result was between 2 to >64 μg/ml.
Cite this paper
Chowdhury, S. A. , Naher, J. , Mamun, A. A. , Khan, R. A. , Ferdous, J. and Sultana, S. (2014). Studies on Antibiotic Sensitivity Pattern of Pseudomonas aeruginosa Isolated from Hospitalized Patients. Open Access Library Journal, 1, e911. doi: http://dx.doi.org/10.4236/oalib.1100911.
Brooks, G.F., Butel, J.S. and Moore, S.A. (2004) Jawetz, Melnick and Adelberg’s Medical Microbiology. 23rd Edition, McGraw-Hill/Appleton and Lange, New York.
Rayner, C.F.J., Cole, P.J. and Wilson, R. (1994) The Management of Chronic Bronchial Sepsis Due to Bronchiectasis. Clinical Pulmonary Medicine, 1, 348-355.
http://dx.doi.org/10.1097/00045413-199411000-00002
Seol, B., Naglic, T., Madic, J. and Bedekovic, M. (2002) In Vitro Antimicrobial Susceptibility of 183 Pseudomonas aeruginosa Strains Isolated from Dogs to Selected Antipseudomonal Agents. Journal of Veterinary Medicine, 49, 188- 192.
http://dx.doi.org/10.1046/j.1439-0450.2002.00548.x
Li, X.Z., Livermore, D.M. and Nikaido, H. (1994) Role of Efflux Pump(s) in Intrinsic of Pseudomonas aeruginosa: Resistance to Tetracycline, Chloramphenicol and Norfloxacin. Antimicrob. Antimicrobial Agents and Chemotherapy, 38, 1732-1741.
http://dx.doi.org/10.1128/AAC.38.8.1732
Schaber, J.A., Carty, N.L., MacDonald, N.A., Graham, E.D., Cheluvappa, R., Griswold, J.A. and Hamood, A.N. (2004) Analysis of Quorum Sensing-Dificient Clinical Isolates of Pseudomonas aeru-ginosa. Journal of Medical Microbiology, 53, 841-853.
http://dx.doi.org/10.1099/jmm.0.45617-0
(2003) National Committee for Clinical Laboratory Standards, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard M7-A6, National Committee for Clinical Laboratory Standards, Wayne.
National Committee for Clinical Laboratory Standards, Performance Standards for Anti-microbial Susceptibility Testing (2003) M100-S13. National Committee for Clinical Laboratory Standards, Wayne.
Kumar, A. and Talwar, A. (2010) Study on the Presence of K. pneumonia and K. oxytocain Raw Milk and Antibiotic Susceptibility Testing. Human Ecology, 32, 207-208.
Visser, M.R., Arska, M.R., Beumer, H., Hoepelman, I.M. and Verhoef, J. (1991) Comparative in Vitro Antibacterial Activity of Sparfloxacin (AT-4140; RP 64206), a New Quin-olone. Antimicrobial Agents and Chemotherapy, 35, 858- 868.
http://dx.doi.org/10.1128/AAC.35.5.858
Calva, J.J., Sifuentes-Osornio, J. and Ceron, C. (1996) Antimicrobial Resistance in Fecal Flora: Longitudinal Community-Based Surveillance of Children from Urban Mexico. Antimicrobial Agents and Chemotherapy, 40, 1699-1702.
Sack, R.B., Rahman, M., Yunus, M. and Khan, E.H. (1997) Antimicrobial Resistance in Organisms Causing Diarrheal Disease. Clinical Infectious Diseases, 24, S102-S105.
http://dx.doi.org/10.1093/clinids/24.Supplement_1.S102
Hoge, C.W., Gambel, J.M., Srijan, A., Pitarangsi, C. and Echeverria, P. (1998) Trends in Antibiotic Resistance among Diarrheal Pathogens Isolated in Thailand over 15 Years. Clinical Infectious Diseases, 26, 341-345.
http://dx.doi.org/10.1086/516303
Carmeli, Y., Troillet, N., Eliopoulos, G.M. and Samore, M.H. (1999) Emergence of Antibiotic-Resistant Pseudomonas aeruginosa: Comparison of Risks Associated with Different Antipseudomonal Agents. Antimicrobial Agents and Chemotherapy, 43, 1379-1382.
Lee, P.R., Lurie, P., Silverman, M.M. and Lydecker, M. (1991) Drug Promotion and Labeling in Developing Countries: An Update. Journal of Clinical Epidemiology, 45, 49-55.
http://dx.doi.org/10.1016/0895-4356(91)90113-N
Paredes, P., Pena, M., Guerra, E.F., Diaz, J. and Trostle, J. (1996) Factors Influencing Physicians’ Prescribing Behavior Ion the Treatment of Childhood Diarrhea: Knowledge May Not Be the Clue. Social Science & Medicine, 42, 1141-1153.
http://dx.doi.org/10.1016/0277-9536(95)00387-8