The purpose of this study was to
estimate relationship between and within some Sudanese camel breeds (Kababeish,
Shanabla and Nyalawei) by digestion of mitochondrial DNA using three different
restriction enzymes. DNA was extracted from 45 blood samples of Sudanese camels
(15 form each breed). Polymerase chain reaction was done using specific primers
in order to amplify the D-Loop region. The PCR products were digested using 3
different restriction enzymes (tag1, hinf and scal). The products were run on
agarose gel 2%. The result of this study revealed differences between the three
breeds according to digested and undigested samples. Tag1 did not cut any of
the 45 samples of all breeds, while scal cut 4.4% of the Shanabla breed, 6.7%
of Kababeish breed, but did not cut any of Nyalawei camels. The third
restriction enzyme hinf cut all sample from Shanabla camels, and also cut 95.6%
of Kababeish breed and also 95.6% of Nyalawei camels.
Cite this paper
Sulieman, M. , Malik, Y. A. , Ahmed, S. S. , Makkawi, A. A. and Gornas, N. (2014). Digestion of Mitochondrial DNA of Some Sudanese camel breeds using three different Restriction Enzymes. Open Access Library Journal, 1, e667. doi: http://dx.doi.org/10.4236/oalib.1100667.
Toro, M.A., Fernandez, J. and Caballero, A. (2006) Scientific Basis for Policies in Conservation of Farm Animal Genetic Resources. Proceedings of 8th World Congress on Genetics Applied to Livestock Production, Belo Horizonte, United Nations, Rome.
Hanotte, O. and Jianlin, H. (2005) Genetic Characterization of Livestock Populations and Its Use in Conservation Decision Making. Proceedings of Workshop on the Role of Biotechnology, Turin, 5-7 March 2005, 131-136.
Alvarez, I., Traore, A., Tamboura, H.H., Kabore, A., Royo, L.J., Fernandez, I., Ouédraogo-Sanou, G., Sawadogo, L. and Goyache, F. (2009) Microsatellite Analysis Characterizes Burkina Faso as a Genetic Contact Zone between Sahelian and Djallonke Sheep. Animal Biotechnology, 20, 47-57. http://dx.doi.org/10.1080/10495390902786926
Tapio, I., Tapio, M., Grislis, Z., Holm, L.E., Jeppsson, S., Kantanen, J., Miceikiene, I., Olsaker, I., Viinalass, H. and Eythorsdottir, E. (2005) Unfolding of Population Structure in Baltic Sheep Breeds Using Microsatellite Analysis. Heredity, 94, 448-456. http://dx.doi.org/10.1038/sj.hdy.6800640
Ishida, N., Hasegawa, T., Takeda, K., Sakagami, M., Onishi, A., Inumaru, S., Kamtsu, M. and Mukoyama, H. (1994) Polymorphic Sequence in the D-Loop Region of the Equine Mitochondrial DNA. Animal Genetics, 25, 215-221. http://dx.doi.org/10.1111/j.1365-2052.1994.tb00196.x
Alvarez, I., Gutierrez, J.P., Royo, L. J., Fernandez, I., Gomez, E., Arranz, J.J. and Goyache, F. (2005) Testing the Usefulness of the Molecular Coancestory Information to Assess Genetic Relationships in Livestock Using a Set of Spanish Sheep Breeds. Animal Science, 83, 737-744.
Aquadro, C.F., Jennings, R.M., Bland, M.M., Laurie, C.C. and Langley, C.H. (1992) Patterns of Naturally Occurring Restriction Map Variation, Dopa Decaoxylase Activity Variation and Linkage Disequilibrium in DdC Gene Region of Drosphilia melongaster. Genetics of American Society, 132, 443-452.
El-Morshedy, A.E.-D., El-Daly, E.-S.A., El-Atabany, A.I. and Tharwat, A.E. (2011) Identification of Adulteration with Camel Meat Using Polymerase Chain Reaction Assay. Journal of American Sciences, 7, 339-343.