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Alteraciones del mecanismo de la fagocitosis en el paciente politraumatizado
Sin Mayor,Adriana; Castellanos Puerto,Edelis; Rodríguez Acosta,Mireida; Vázquez González,Tatiana; Jonhston Dreke,Noralba; Rojas Moya,Ana;
Revista Cubana de Medicina Militar , 2000,
Abstract: a phagocytic assessment of polymorphonuclear neutrophils were carried out in 22 multitrauma patients and 22 healthy subjects in the immunology laboratory of "dr luis díaz soto" higher institute of military medicine. opsonophagocytic index was measured in all the participants in the study and opsonized yeasts were subjected to polymorphonuclear leukocytes. the objective of the evaluation was to prove whether there were significant differences between the groups. a marked reduction of phagocytic function was observed in trauma patients compared with healthy subjects and with the reference values. it was demonstrated that trauma produces massive deterioration and impaired immunological activity.
PKC Is a Target to Modulate the Expression of Receptor Mediated Endocytosis (RME) Mice Macrophages BALB/c for Optimizing the Phagocytosis toward Candida albicans  [PDF]
Adi Prayitno, Elyana Asnar, Okid Parama Astirin, Anief Nur Artanti, Meutia Srikandi Fitria, Eva Agustina Perwitasari, Suhartono Taat Putra
Journal of Immune Based Therapies, Vaccines and Antimicrobials (JIBTVA) , 2013, DOI: 10.4236/jibtva.2013.24006
Abstract:


Introduction: The existence of receptor-mediated endocytosis (RME) means that selectivity and selectivity occurs in capturing macromolecules. Protein kinase C (PKC) which can be expressed by almost all cells are proteins important in signal transduction groove that plays a role in a number of cell activity, e.g. phagocytosis. Aims: The purpose of this study is to determine the expression of RME after modulating the PKC which is characterized by the number of Candida albicans cells attached to the surface of macrophages. Methods: Peritoneal macrophages cultured BALB/c mice are treated with PMA and/or bisindolylmaleimides of providing levels of 5 ng/ml to 100 ng/ml for 10 minutes. Then immediately insert Candida albicans and observe every 30 minutes for 120 minutes. The research design used the same subject. Data collected in the form of number of Candida albicans cells attached to the surface of macrophages are analyzed with ANOVA statistical test (one way) to show the differences between treatments. Results: The test shows statistically significant difference in the number of Candida albicans cells attached to the surface of macrophages after administration of various levels of PMA (p < 0.001). The higher level of PMA is given, the more active the PKC is

Defectos en la fagocitosis: Aspectos clínicos, moleculares y terapéuticos
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2004,
Abstract: a review is made on the defect present in the phagocytes’ function that cause immunity disorders. the clinical, molecular and immunological characteristics of each phagocytic disorder, as well as the laboratory methods used for their diagnosis are exposed. reference is made to the effective therapeutic treatments that erradicate or alliviate the clinical manifestations of every studied defect of phagocytosis
Defectos en la fagocitosis: Aspectos clínicos, moleculares y terapéuticos Defects in phagocytosis. Clinical, molecular and therapeutic aspects
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2004,
Abstract: A review is made on the defect present in the phagocytes’ function that cause immunity disorders. The clinical, molecular and immunological characteristics of each phagocytic disorder, as well as the laboratory methods used for their diagnosis are exposed. Reference is made to the effective therapeutic treatments that erradicate or alliviate the clinical manifestations of every studied defect of phagocytosis
A digital cmos sequential circuit model for bio-cellular adaptive immune response pathway using phagolysosomic digestion: a digital phagocytosis engine  [PDF]
Sayed Mohammad Rezaul Hasan
Journal of Biomedical Science and Engineering (JBiSE) , 2010, DOI: 10.4236/jbise.2010.35065
Abstract: Living systems have to constantly counter micro-or- ganisms which seek parasitic existence by extracting nutrition (amino acids) from the host. Phagocytosis is the ingestion of micro-creatures by certain cells of living systems for counter nutrition (breakdown of the micro-creature into basic components) as part of cellular adaptive immune response. These particular cells are called phagocytes, all of which are different types of white blood cells or their derivatives. Phagocytes are activated by certain components of the micro-creatures which act as an antigen, generating an- tibody secretion by the phagocyte. This paper develops a digital CMOS circuit model of phagocytosis: the immune response biochemical pathway of a pha- gocyte. A micro-sequenced model has been developed where the different stages in phagocytosis are modeled as different states clocked by circadian time intervals. The model converts the bio-chemical immune system digestive pathway into a cascade of CMOS multi-step logical transformations from micro-crea- ture ingestion to the secretion of indigestible residuals. This modeling technique leads to the understanding of cellular immune deficiency diseases of living systems in the form of logical (electrical) faults in a circuit.
Proposal for a new evaluation of phagocytosis using different sizes of fluorescent polystyrene microspheres  [PDF]
Riyo Enomoto, Makoto Imamori, Ayoumi Seon, Kozue Yoshida, Aya Furue, Hirofumi Tsuruda, Eibai Lee-Hiraiwa
Advances in Biological Chemistry (ABC) , 2013, DOI: 10.4236/abc.2013.36064
Abstract:

To investigate phagocytosis, peritoneal-resident and J774.1 macrophages were incubated with fluorescent polystyrene microspheres measuring 1.0 μm in diamter at 200 particles per cell. The amount of phagocytized microspheres increased with incubation time, and both cell types had similar phagocytic activity. Further, we investigated the phagocytosis of different sizes of microspheres by J774.1 macrophages. To adequately evaluate phagocytosis, varying amounts of different sizes of microspheres were added to J774.1 cells, and their phagocytic activities were evaluated. When the microspheres were added at a density of 20 particles per cell, few small microspheres (<1.0 μm in diameter) were phagocytized. This result suggested that their low amount caused difficulty in evaluating phagocytosis. In contrast, when the same variety of microspheres was added at a density of 200 particles per cell, phagocytosis of large microspheres (>3 μm in diameter) could not be evaluated because of cytotoxicity. Thus, the amount of different sizes of microspheres added is important for precisely evaluating phagocytic activity. When the amount of different sizes of microspheres added was standardized to provide a set amount of total surface area, phagocytosis of these microspheres could be adequately evaluated and compared. To determine the effects of phagocytosis on cell viability and proliferation, cells incubated with different sizes of microspheres were assayed using a cell counting kit. We found that phagocytosis had no effect on cell viability or proliferation and was independent of particle size. Furthermore, cells already phagocytized microspheres retained their phagocytic activity.

 

Phosphatidylserine receptor and apoptosis: consequences of a non-ingested meal
Marina Botto
Arthritis Research & Therapy , 2004, DOI: 10.1186/ar1191
Abstract: Apoptosis is an active process of cell suicide that leads to ordered destruction of the cells and their safe disposal by professional (macrophages and immature dendritic cells) and nonprofessional (such as fibroblasts and epithelial cells) phagocytes [1]. The removal of apoptotic cells is the final step and perhaps the ultimate objective of the apoptotic programme. When apoptosis was initially described by Kerr and coworkers [2], the phenomenon of programmed cell death was greeted with a striking lack of interest. It has now become apparent that the process is ubiquitous and plays a key role in many fundamental biological events, including embryonic development, normal tissue homeostasis, development of the immune system and resolution of inflammation. In addition, apoptotic cells are a potential source of self-antigens [3], and defective clearance of cell corpses has recently been implicated in the pathogenesis of autoimmune diseases [4].Although enormous progress has been made in our understanding of the molecular mechanisms of apoptosis, the events that lead to clearance of apoptotic cells are still undefined. However, it has become increasingly clear that in vivo apoptosis and engulfment are not distinct events, but rather are two linked stages in the same process. Cells dying by apoptosis provide both 'recruitment' and 'eat me' signals to scavenger cells, facilitating their own uptake [5]. The best studied of these signals is exposure of phosphatidylserine (PS), a phospholipid that is normally limited to the inner leaflet of the plasma membrane bilayer. Although it has been well established that PS exposure on apoptotic cells is critically required for their proper uptake, the identification of a single dominant receptor that is capable of recognizing and removing the apoptotic cells has remained controversial.Many receptors and soluble ligands have been proposed to mediate the recognition and uptake of apoptotic cells. These include lectin-like molecules, scav
Effects of life events and stress on neutrophil functions in elderly men
Kazumasa Tsukamoto, Kazuhiko Machida
Immunity & Ageing , 2012, DOI: 10.1186/1742-4933-9-13
Abstract: We investigated the relationships between neutrophil function measured using the nitroblue tetrazolium (NBT) reduction test and measurements of psychological stress and life events among 81 men aged over 60?years.The numbers and scores for life events were significantly higher (p?<?0.01, respectively) in a group with values reflecting phagocytosis below the median than in a control group.Chronic psychological stress due to life events decreases neutrophil functions among elderly men.
Tempo de migra??o dos macrófagos em matrinx?, Brycon amazonicus, por meio da técnica de inocula??o de leveduras Saccharomyces cerevisiae
Dias, Danielle de Carla;Tachibana, Leonardo;Seriani, Robson;Santos, Antenor Aguiar;Ranzani-Paiva, Maria José Tavares;Romagosa, Elizabeth;
Acta Amazonica , 2011, DOI: 10.1590/S0044-59672011000300013
Abstract: in aquaculture, analysis of activation and increased macrophages migration are used in order to verify the ability of the nonspecific immune fish exposed to a challenge. this study aimed to determine the time of macrophages migration in matrinx?, brycon amazonicus, through the technique of yeast saccharomyces cerevisiae inoculation, verifying possible changes in hematological parameters. thirty animals with average weight of 101.55 ± 24.50 g and average length of 19.75 ± 1.72 cm were employed. the experimental inoculation periods were 2, 4, 8 and 12 hours. thereafter, animals were anesthetized and blood was withdrawn through a caudal puncture for the determination of total erythrocytes number, differential and total leukocyte counts and total thrombocytes count, hematocrit, hemoglobin concentration and calculation of the erythrocytes index. the results for the phagocytic capacity were not significantly different between experimental periods. in the phagocytic index, the period of 2 hours presented the highest rate of phagocytized cells, indicating that 2 hours of incubation was sufficient for the macrophages migration in b. amazonicus. the number of erythrocyte was the only parameter that presented significant difference among periods.
Capacidade fagocitária de ratos esplenectomizados
Biondo-Sim?es, Maria de Lourdes Pessole;Pante, M?nica Lidia;Liberato, Carla Cristina Gularte;Gauginski Jr, José Ciro;Macedo, Vanesa Lara de;Dias, Cristiane;
Acta Cirurgica Brasileira , 2000, DOI: 10.1590/S0102-86502000000700004
Abstract: the spleen, during a long time was considered an organ without a definite function. nowadays it is considered an elaborate defense system, mainly of phagocitosis. the present study intends to present the influence of total splenectomy on total phagocytic capacity. twenty-six male mice were used, 180 days old with medium weight of 289.5 g, which were divided in 3 groups: control ( n=10), sham (n=8) and experiment (n=8). the animals from control group were used to provide the phagocytosis standard. the sham group were submitted to median laparotomy under general anesthesia with peritoneal sodium-thiopental. the experiment group suffered the same process and total splenectomy. after 7 days, blood samples were taken and iron solution was injected (2mg/kg). new blood samples were collected after 5 minutes, and 15 minutes of injection. the serum iron from the samples were dosed and the phagocytosis index were calculated from the equation of biozzi et al. the phagocytosis index were similar in control and sham groups (p=0,143086) and lower in experiment group (p=0,000685). the total phagocytic capacity is diminished after splenectomy in rats.
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