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This study proposes the development of contamination measurement for industrial machines based on magnetic field method. A permanent magnet was used to generate the 0.17 T magnetic fields and Hall Effect sensor was applied to measure the contamination level of metal particles in industrial machine lubricant during operation time and to predict the replacement period if the lubricant condition does not meet the standard (NAS 1638). Contamination level of metal particles was directly related to the output voltage of Hall Effect sensor. When there was any movement of metal particles to the permanent magnet, the measured result was then transferred to a computer for analysis. This methodology could be applied to experiment the conditions of used lubricant for assessing the contamination and the metal particle remaining in the tank or inside the pipe of the machine as well as the conditions of car lubricant and other lubricants used in industrial processes.
The purification of a lipase isoenzyme from an Aspergillus niger lipase A is reported in this manuscript.
Purification was carried out in a simple adsorption step, in which the lipase
was offered at low ionic strength to the commercially available C8 modified
magnetic particles, MaKProt C8. When the isoenzyme was desorbed with a 0.2%
solution of Triton X-100, the SDS-PAGE gel showed a single pure band with a
molecular weight of 35 KDa. The purified fraction showed 66.75-fold purification
compared with the crude extract. The pure fraction was characterized along with
the crude extract and the lipase adsorbed on the MaKProt C8. The purified and
the adsorbed lipase showed better activity for the tested substrates
(p-nitrophenyl acetate, decanoate, myristate and palmitate) than the crude extract, the preferred substrates being myristate
(26.7 μmol·min-1·mg-1) and decanoate (17.4 μmol·min-1·mg-1),
respectively. The temperature and pH profiles showed no change for the three
enzymes, the optimum temperature being 37°C and the best pH 7.0.