oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 1 )

2019 ( 6 )

2018 ( 14 )

2017 ( 14 )

Custom range...

Search Results: 1 - 10 of 837 matches for " demembranated sperm "
All listed articles are free for downloading (OA Articles)
Page 1 /837
Display every page Item
Nuclear reconstitution of demembranatedOrychophragmus violaceus sperm inXenopus laevis egg extracts
Ping Lu,Min Ren,Zhonghe Zhai
Science China Life Sciences , 2002, DOI: 10.1007/BF02879750
Abstract: The cell-free extracts from animalXenopus laevis egg could induce chromatin decondensation and pronuclear formation from demembranated plant (Orychophragmus violaceu) sperm. The demembranatedOrychophragmus violaceus sperm began to swell in 30 min incubation, and then were gradually decondensed. The reassembly of nuclear envelope in the reconstituted nuclei had been visualized by means of electron microscopy and fluorescent microscopy. Membrane vesicles fused to form the double envelope around the periphery of the decondensed chromatin. The morphology of the newly formed nucleus, with a double membrane, was similar to those nuclei after fertilization. Transmission electron microscope micrograph of the whole mount prepared nuclear matrix-lamina showed the reconstituted nucleus to be filled with a dense network.
Nicotiana ovule extracts induce nuclear reconstitution of demembranated Xenopus sperm in cell-free system
Ping Lu,Min Ren,Zhonghe Zhai
Chinese Science Bulletin , 2002, DOI: 10.1360/02tb9072
Abstract: Nicotiana tabaccum ovule extracts induced nuclear reconstitution of demembranated Xenopus leavis sperm in a cell-free system. Demembranated Xenopus sperm began to swell after 15 min of incubation with Nicotiana ovule extracts. Accompanying the process of incubation, Xenopus sperm decondensed and their shapes changed gradually from long and ellipse to round. The completely decondensed chromatin was surrounded with membrane structure, which was a mixture envelope of a double membrane and a single membrane. Nucleosome assembly was verified by means of micrococcal nuclease digestion to reconstituted nuclei and DNA electrophoresis. Nicotiana ovule extracts supplied one more experimental model and system. The new system could promote powerfully the research on mechanisms of cell division and cell cycle regulation.
Nuclear reconstitution of demembranatedOrychophragmus violaceus sperm inXenopus laevis egg extracts

Ping Lu,Min Ren,Zhonghe Zhai,

中国科学C辑(英文版) , 2002,
Abstract: The cell-free extracts from animalXenopus laevis egg could induce chromatin decondensation and pronuclear formation from demembranated plant (Orychophragmus violaceu) sperm. The demembranatedOrychophragmus violaceus sperm began to swell in 30 min incubation, and then were gradually decondensed. The reassembly of nuclear envelope in the reconstituted nuclei had been visualized by means of electron microscopy and fluorescent microscopy. Membrane vesicles fused to form the double envelope around the periphery of the decondensed chromatin. The morphology of the newly formed nucleus, with a double membrane, was similar to those nuclei after fertilization. Transmission electron microscope micrograph of the whole mount prepared nuclear matrix-lamina showed the reconstituted nucleus to be filled with a dense network.
Nicotiana ovule extracts induce nuclear reconstitution of demembranated Xenopus sperm in cell-free system

Ping Lu,Min Ren,Zhonghe Zhai,

科学通报(英文版) , 2002,
Abstract: Nicotiana tabaccum ovule extracts induced nuclear reconstitution of demembranated Xenopus leavis sperm in a cell-free system. Demembranated Xenopus sperm began to swell after 15 min of incubation with Nicotiana ovule extracts. Accompanying the process of incubation, Xenopus sperm decondensed and their shapes changed gradually from long and ellipse to round. The completely decondensed chromatin was surrounded with membrane structure, which was a mixture envelope of a double membrane and a single membrane. Nucleosome assembly was verified by means of micrococcal nuclease digestion to reconstituted nuclei and DNA electrophoresis. Nicotiana ovule extracts supplied one more experimental model and system. The new system could promote powerfully the research on mechanisms of cell division and cell cycle regulation.
Evidence for the involvement of NGF in human sperm motility  [PDF]
Cui-Ge Shi, Kai Lin, Xiang-Bo Xu, Shu-Cheng Zhang, Ning Wang, Ming Fan
Journal of Biomedical Science and Engineering (JBiSE) , 2012, DOI: 10.4236/jbise.2012.59066
Abstract: Motility is an important physiological characteristic of a mature sperm. Nerve growth factor (NGF) is a key protein for the survival, maintenance and development of the central and peripheral nervous systems. It has been shown that NGF and its receptors TrkA and p75 are widely expressed in the testis, accessory reproductive organ, and the epididymal sperms. These observation have shifted the attention to the role of NGF on male reproductive physiology. In the present study, we found that NGF remarkably increased testicular coefficient in rats subjected to unpredictable chronic mild stress. Furthermore, we investigated the role of NGF on human sperm motility in vitro by CASA. The results showed that the parameters of sperm motility after NGF treatment had significantly increased, the means of VAP, VSL, VCL, BCF and LIN were significantly increased 32% than those of NGF absence, the means of MAD, STR, ALH and WOB had no notable difference. In addition, NGF promotes the sperm motility in a time- and dose-dependent manner. Taken together, our findings suggest that NGF plays a promoted role in sperm motility.
Effects of Sertraline on Sperm Motility, Number and Viability and Its Relation to Blood Levels of Testosterone, FSH and LH in Adult Male Mice  [PDF]
Mahsa Hadipour Jahromy, Anousheh Amini Moghadam
Advances in Sexual Medicine (ASM) , 2014, DOI: 10.4236/asm.2014.42004
Abstract: The aim of this study was to investigate the effects of Sertraline on gonad-pituitary cycle (Testosterone, FSH and LH) and reproductive cells in adult male Balb/C mice. Adult male Balb/C mice were divided into the following 5 groups: control group with no treatment, sham group which received solvent, and the experimental groups which received one dose of Sertraline (0.0178 mg/kg) in the first group, two doses of Sertraline (0.0356 mg/kg) in the second group, and three doses of Sertraline (0.0534 mg/kg) in the third group. Mice were anaesthetized after 7 weeks. Serum samples were collected and Testosterone, FSH and LH levels of serum were assayed. Vase deferans were analyzed for motility, number and viability of sperms. The results of this study showed that the viability, count and motility of sperms were decreased. Testosterone level of blood was also decreased while FSH was increased. There was no significant change in LH level. It is suggested that Sertraline at higher dose decreases sperm production and has the potential to affect adversely fertility in male mice.
Diagnostic and Analysis of Human Sperm Characteristics Using Fourier Transform Infrared Spectroscopy  [PDF]
Amir Abramovich, Alexander Shulzinger
Open Journal of Urology (OJU) , 2015, DOI: 10.4236/oju.2015.56015
Abstract: A spectroscopic method for human sperm evaluation and characterization using Fourier Transform Infra Red (FTIR) is presented. The high sensitivity of FTIR to changes in chemical structure and arrangement of molecules and proteins makes it a powerful diagnostic tool. Our experimental results show that a simple MIR (400 cm-1?- 4000 cm-1) transmission spectrum of a human sperm is very fast and can be used to determine the level of structure, compare to conventional LAB tests. No sample preparations are required, the semen has to be put on a special ZnSe substrate and inserted into the measurement compartment of the FTIR. Furthermore, this method can distinguish between immature sperm cell to white blood cell which by using a microscope is difficult and re-quires experience.
Association of Osteopontin Gene Promoter Single Nucleotide Polymorphisms with Bull Semen Quality  [PDF]
Rick W. Rorie, Chance L. Williams, Toby D. Lester
Advances in Reproductive Sciences (ARSci) , 2016, DOI: 10.4236/arsci.2016.41001
Abstract: Osteopontin (OPN) is a protein found at higher concentrations in the seminal plasma of bulls with above average fertility. Polymorphisms have been reported within the OPN gene promoter that can affect production of this protein and thus, affect fertility. Therefore, Angus (n = 5) and Angus x Gelbvieh (Balancer, n = 14) and Angus x Brahman (n = 15) bulls were evaluated for presence of single nucleotide polymorphisms (SNP) in the Bos taurus OPN gene (GenBank: AY878328.1) promoter region, and their possible effects on bull semen quality as evaluated by computer-assisted semen analysis (CASA). Semen was collected by electroejaculation 6 to 9 times from each bull, and each semen collection was evaluated by CASA for motile, progressive and rapid sperm within 5 mins of ejaculation. The bulls were genotyped for reported single nucleotide polymorphisms (SNP) in the promoter region of the OPN gene through amplification of two 700 base pair (bp) DNA fragments and sequencing of the resulting PCR products. Seven SNP sites were identified, at bp 3379, 3490, 3492, 5075, 5205, 5209, and 5263 of the OPN gene. The SNP identified at bp 5205, 5209 and 5263 had not been previously reported. Individual SNP sites were evaluated as the main effect on CASA sperm motility variables in a SAS MIXED model for repeated measures. A thymine to guanine substitution at bp 3379 was associated with increased (P ≤ 0.02) percentage of motile, progressive and rapid sperm in Angus x Brahman bulls, and tended (P ≤ 0.10) to increase the same sperm motility parameters in Angus, and Angus x Gelbvieh bulls. The percentages of motile, progressive and rapid sperm were similar (P ≥ 0.05) among genotypes for the other 6 SNP identified. These results suggest that identification and genotyping of polymorphisms within the promoter region of the bovine OPN gene may be useful for selecting bulls with improved sperm motility parameters.
Effect of age on semen parameters in infertile men after varicocelectomy
Hassanzadeh-Nokashty K, Yavarikia P, Ghaffari A, Hazhir S, Hassanzadeh M
Therapeutics and Clinical Risk Management , 2011, DOI: http://dx.doi.org/10.2147/TCRM.S17027
Abstract: t of age on semen parameters in infertile men after varicocelectomy Original Research (2445) Total Article Views Authors: Hassanzadeh-Nokashty K, Yavarikia P, Ghaffari A, Hazhir S, Hassanzadeh M Published Date August 2011 Volume 2011:7 Pages 333 - 336 DOI: http://dx.doi.org/10.2147/TCRM.S17027 Kamaleddin Hassanzadeh-Nokashty1, Parisa Yavarikia2, Alireza Ghaffari3, Samad Hazhir1, Mohammadali Hassanzadeh1 1Department of Urology, 2Faculty of Nursery and Midwifery, 3Department of Internal Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Background: The effectiveness of varicocelectomy in restoration of fertility and improvement of semen parameters is still controversial. The purpose of this study was to determine the effect of age on semen parameters following varicocelectomy in a group of infertile men. Methods: Improvements in sperm count, morphology, and motility were studied in 67 infertile patients 4–10 months after varicocelectomy. Results: The mean age of the patients was 30.48 ± 7.49 years. Significant improvements in total sperm count, percentage normal morphology, and motility were noted in all age groups (P < 0.05). Patients aged <25 years demonstrated the greatest increase in sperm counts, normal morphology, and motility following varicocelectomy. There was a significant negative correlation between age and sperm count, sperm morphology, and sperm motility (P < 0.05). Conclusion: The effect of age on improvement in sperm parameters after varicocelectomy is inconsistent with some reports in the literature, and could be attributable to the duration of infertility prior to surgery; in the long term, varicoceles are known to have deleterious effects on testis biology.
Effects of Different Doses of Hyaloronan on Human Sperm Motility, Vitality and Morphology
Mohammadi Roushandeh Amaneh,Sohrabvand Farnaz,Amanpour Saeid,Pasbakhsh Parichehr
Acta Medica Iranica , 2009,
Abstract: Important aspect of sperm function such as motility and capacitation appear to be mediated at least partially though hyaloronic acid (HA). Present study investigated effects of different doses of HA on sperm motility and vitality in human. Sperm was obtained from 20 male from IVF clinic in Imam Khomeini Hospital. Sperm motility and vitality in human semen was analyzed according to WHO criteria before and 4 hours after treatment with different doses of HA (0.750, 1000 and 1250 μg/ml). The results showed that in 1000 μg/ml the percent of stage 3 and 4 increased compare to control group. Percent of stage 1 and 2 decreased in group with 1000 μg/ml HA, there was an increase in the percentage of stage 3 and 4 and decrease in percentage of stage 1 and 2 compare to control. In the group treated with 1250 μg/ml stage 1 and 2 increased while stage 3 and 4 decreased. Vitality in all groups decreased except of the group treated with 1000 μg/ml HA. The group with 1250 μg/ml showed significantly decrease in vitality compare to fresh group (P < 0.05). The present study showed that the effects of HA on sperm motility and vitality is dose dependant and 1000 μg/ml HA had the effective role on sperm parameters.
Page 1 /837
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.