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Search Results: 1 - 10 of 364574 matches for " cytochrome b559<br>D1蛋白质 "
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The presence of phosphorylation form of D1 protein in its cross-linked aggregates in high light treated spinach leaves in vivo
Huimin Wei,Junwei Guo,Shuang Zhang,Bo Huang,Yinqiu Liu,Linfang Du,
WEI
,Huimin,GUO,Junwei,ZHANG,Shuang,HUANG,Bo,LIU,Yinqiu,DU,Linfang

科学通报(英文版) , 2006,
Abstract: In the present study, using specific antibody against D1 protein, we detected four aggregates of D1 protein in thylakoid membranes from spinach leaves illuminated at high light (800–2500 μmol photons·m 2·s 1) for 3 h. Their accumulations were dependent on the light intensity to which the leaves had been subjected. Further immunoblot analysis indicated that 70 kD aggregate was a product of D1 protein cross-linked with CP43, 65 and 60 kD aggregate were two cross-linked products between D1 and D2 proteins, and 41 kD aggregate was one cross-linked D1 with α-subunit of cytochrome b 559 (Cyt b 559). This result provided the evidence for the existence of the aggregation of the D1 protein in vivo. The maximal level of D1/Cyt b 559 aggregate occurred at 1000 μmol photons·m 2·s 1 but drastically decreased with further increasing light intensity. Immunoblot analysis with phosphothreonine (Thr (P)) antibody indicated that D1/CP43 and D1/Cyt b 559 aggregates contained the phosphorylated protein(s). In vitro dephosphorylation experiment also showed that D1/Cyt b 559 and D1/CP43 aggregates lost the immunoreactivity with Thr (P) antibody after the phosphatase treatment of the membranes from high-light-illuminated leaves. Our results demonstrated that strong illumination of spinach leaves induced cross-linked products of D1 protein with its nearby polypeptides of PS, some of which co.n-tained the phosphorylated D1 protein.
强光照处理菠菜叶片产生的交联聚合物中D1蛋白部分发生磷酸化
魏慧敏,郭军伟,张爽,黄博,刘映秋,杜林方
科学通报 , 2005, DOI: 10.1360/972005-1529
Abstract: 利用D1蛋白特异性抗体,在强光照(800~2500μmolphotons·m-2·s-1)处理3h的菠菜叶片类囊体膜中检测到4种D1蛋白聚合物,它们的相对含量与处理时光照强度相关.进一步分析表明,70kD聚合物为D1蛋白与CP43交联产物,65和60kD聚合物是D1蛋白与D2蛋白交联产物,41kD聚合物是D1蛋白与细胞色素b559α亚基交联物.1000μmolphotons·m-2·s-1光照处理生成的D1/Cytb559聚合物最多,光强增加时其含量下降.磷酸化苏氨酸抗体Westernblot分析结果显示,D1/Cytb559和D1/CP43聚合物中存在磷酸化蛋白;外加磷酸酯酶处理后,磷酸化的D1/Cytb559和D1/CP43聚合物减少.上述结果表明,强光照处理叶片引起D1蛋白与其他PSⅡ核心蛋白交联,所生成的聚合物中部分D1蛋白以磷酸化形式存在.
psⅱ反应中心d1蛋白的小肽抗体的制备和鉴定
李晓鹏?,杜林方?,梁厚果?,吴宛荪?
生物化学与生物物理进展 , 1997,
Abstract: 用人工合成的psⅱ反应中心d1蛋白中(229~240)的12肽,与牛血清白蛋白偶联后做为抗原免疫家兔,获得抗菠菜d1蛋白抗体.免疫双扩散法测得其具较高的效价,蛋白印迹检测结果显示该抗体仅与d1蛋白有免疫亲和反应.表明此抗体可作为检测d1蛋白及其光抑制时降解产物的探针.
The presence of phosphorylation form of D1 protein in its cross-linked aggregates in high light treated spinach leaves in vivo
Huimin Wei,Junwei Guo,Shuang Zhang,Bo Huang,Yinqiu Liu,Linfang Du
Chinese Science Bulletin , 2006, DOI: 10.1007/s11434-005-1529-3
Abstract: In the present study, using specific antibody against D1 protein, we detected four aggregates of D1 protein in thylakoid membranes from spinach leaves illuminated at high light (800–2500 μmol photons·m 2·s 1) for 3 h. Their accumulations were dependent on the light intensity to which the leaves had been subjected. Further immunoblot analysis indicated that 70 kD aggregate was a product of D1 protein cross-linked with CP43, 65 and 60 kD aggregate were two cross-linked products between D1 and D2 proteins, and 41 kD aggregate was one cross-linked D1 with α-subunit of cytochrome b 559 (Cyt b 559). This result provided the evidence for the existence of the aggregation of the D1 protein in vivo. The maximal level of D1/Cyt b 559 aggregate occurred at 1000 μmol photons·m 2·s 1 but drastically decreased with further increasing light intensity. Immunoblot analysis with phosphothreonine (Thr (P)) antibody indicated that D1/CP43 and D1/Cyt b 559 aggregates contained the phosphorylated protein(s). In vitro dephosphorylation experiment also showed that D1/Cyt b 559 and D1/CP43 aggregates lost the immunoreactivity with Thr (P) antibody after the phosphatase treatment of the membranes from high-light-illuminated leaves. Our results demonstrated that strong illumination of spinach leaves induced cross-linked products of D1 protein with its nearby polypeptides of PS, some of which co.n-tained the phosphorylated D1 protein.
CyclinD1、p21WAF1、p53及Ki-67在肝细胞癌中的表达及与预后的关系
王玉兰,杜经丽,石怀银,郭爱桃,韦立新,赵景民
解放军医学杂志 , 2014,
Abstract: 目的 探讨肝细胞癌(HCC)中cyclinD1、p21、p53及Ki-67蛋白的表达及其与HCC预后的关系。方法 选择2000年1月-2005年1月在解放军总医院行手术切除的80例HCC患者的肝组织标本,采用EliVision法进行cyclinD1、p21WAF1、p53及Ki-67免疫组化染色,分析其表达水平与HCC病理特征的关系,并进行生存分析。结果 CyclinD1、p21WAF1、p53及Ki-67在HCC中的阳性表达率分别为38.8%、40.5%、65.4%及80.0%,均明显高于癌旁肝组织(分别为19.0%、11.5%、0.0%、6.3%,P<0.005)。相关分析显示,cyclinD1阳性表达与核分级呈正相关(P=0.041),p21WAF1及p53阳性表达与肿瘤分化程度(P=0.032、P=0.031)和血管浸润(P=0.036、P=0.011)呈正相关,Ki-67阳性表达与肿瘤分化程度(P=0.004)、核分级(P=0.045)和血管浸润(P=0.001)呈正相关。生存分析显示,cyclinD1及Ki-67高表达者预后较差。Ki-67阳性表达与p53(P=0.000)及p21WAF1(P=0.047)阳性表达有明显相关性,其他各蛋白之间表达无明显相关。Cox回归模型分析显示,肿瘤大小(P=0.042)、肿瘤数目(P=0.004)及血管浸润(P=0.000)为HCC的独立预后因素。结论 CyclinD1、p21WAF1、p53及Ki-67可能参与了HCC的生物学进程;cyclinD1及Ki-67阳性表达对HCC预后的判断有一定价值。
Mutagenesis of Ser24 of cytochrome b 559 α subunit affects PSII activities in Chlamydomonas reinhardtii
Mutagenesis of Ser24 of Cytochrome b559 α Subunit Affects PSII Acitivies in Chlamydomonas reinhardtii

Ma Jingjing,Li Liangbi,Jing Yuxiang,Kuang Tinglun,
MA
,JingJing,LI,LiangBi,JING,YuXiang,KUANG,TingYun

科学通报(英文版) , 2007,
Abstract: In order to study the functions of cytochrome b 559 (Cyt b 559) in photosystem two (PSII) activity, mutant S24F of Chlamydomonas reinhardtii was constructed using site directed mutagenesis, in which Serine24 (Ser24) locating downstream of Histidine23 (His23) in α subunit of Cyt b 559 was replaced by Phenylalanine (Phe). Physiological and biochemical analysis showed that mutant S24F could be grown photoautotrophically or photoheterotrophically. However, their growth rate was slower either on HSM or TAP medium than that of the control; Analysis of PSII activity revealed that its oxygen evolution was about 71% of wild type (WT); The Photochemical efficiency of PSII (F v/F m) of S24F was reduced 0.23 compared with WT; S24F was more sensitive to strong light irradiance than the wild type; Furthermore, SDS-PAGE and Western-blotting analysis indicated that the expression levels of α subunit of Cyt b 559, LHCII and PsbO of S24F were a little less than those of the wild type. Overall, these data suggests that Ser24 plays a significant role in making Cyt b 559 structure maintain PSII complex activity of oxygen evolution although it is not directly bound to heme group. Supported by the National Basic Research Program (973 Program) (Grant No. G1988010100) and the National Natural Science Foundation of China (Grant No. 088121A)
Recombinant PsbF from Synechococcus sp. PCC 7002 forms β: β homodimeric cytochrome b559
Recombinant PsbF from Synechococcus sp. PCC 7002 forms β:β homodimeric cytochrome b559

Xin Yu,Ping Tan,Gaozhong Shen,Jindong Zhao,
YUXin
,TANPing

科学通报(英文版) , 2003,
Abstract: All organisms with oxygenic photosynthesis contain two photosystems: photosystemⅠ(PSⅠ) and photosystem-Ⅱ-(PSⅡ). The minimal photosystem-Ⅱ-particles which are photochemically active contain three subunits: D1, D2 and cytochrome b559 (Cyt b559). The function of Cyt b559 remains unclear. We have successfully overexpressed the psbF gene, encoding the - subunit of Cyt b559, from a marine cyanobacterium Synechococcus sp. PCC 7002 as a fusion gene and obtained a redox-active form of Cyt b559. When the N-terminal GST protein of the fusion gene product was removed with thrombin, the PsbF protein was still redox-active, suggesting that the recombinant PsbF can form dimer in Escherichia coli. The absorption spectra of either the oxidized form or the reduced form of both GST fusion protein and the purified PsbF dimer and the difference spectra between the two forms are the same as that of the Cyt b559 isolated from the higher plants. Redox titration analysis of recombinant PsbF showed that the mid-point redox potential of the recombinant Cyt b559 was approximately 50 mV, which is close to the low potential of Cyt b559. The results are helpful to the understanding of localization and function of Cyt b559 on thylakoid membranes.
PHOTODAMAGE OF PHOTOSYSTEM I REACTION CENTER D_1-D_2-CYTOCHROME B_558 COMPLEX
光系统Ⅱ反应中心D_1-D_2-Cyt b_(559)的光破坏作用研究

Yu Zhenbao Kuang Tingyun Tang Chongqin Zhang QidePeng Dechuan Tang Peiaong,
于振宝
,匡廷云,唐崇钦,张其德,彭德川,汤佩松

生物物理学报 , 1992,
Abstract: Photosystem I reaction center D1-D2-Cytochrome b550 purified from high plant was damaged very easily by illumination. During initial 30S of light treatment, the absorbance increased. Following the process of absorbance increasing, continuing illumination led to the decrease of absorbance, blue-shifting of absorption maximum wavelength at red absorption region. Illumination also gave rise to increase of fluorescence emission intensity and blue-shifting of emission maximum wavelength. These results show that there may be two different processes of photodamaging to photosystem I reaction center and P680 is most sensitive to light treatment.
Molecular dynamics simulation and binding free energy calculations of a selective inhibitor of PTP1B
PTP1B选择性抑制剂的分子动力学模拟及结合自由能计算

FANG Lei,JI Ming-Juan,
方磊
,计明娟

中国科学院研究生院学报 , 2009,
Abstract: As a kind of potential drug for treating the Type II diabetes, the inhibitor of protein tyrosine phosphatase 1B (PTP1B) is an important research field, in which a kind of bidentate inhibitors is the research focus. In this paper, molecular dynamics simulation and MM/GBSA method were employed to calculate the binding free energy of the protein-ligand complexes. The rank of the predicted free energies of the complexes is well consistent with the experiment data. The energy decomposition analysis based on the MM/GBSA also indicates the importance of second binding site, especially the residue ARG24 and ARG254 of PTP1B, in producing selectivity against SHP-2, but not so high selectivity against TCPTP. Also the inhibitor has important interaction with the residue of PHE182 of PTP1B, this is another reason that produces the selectivity against TCPTP. We expect that the information obtained in this work can help to develop potential PTP1B inhibitors with more promising specificity.
Recombinant PsbF from Synechococcus sp. PCC 7002 forms β: β homodimeric cytochrome b559
Xin Yu,Ping Tan,Gaozhong Shen,Jindong Zhao
Chinese Science Bulletin , 2003, DOI: 10.1360/03tb9120
Abstract: All organisms with oxygenic photosynthesis contain two photosystems: photosystem I (PS I) and photosystem II (PS II). The minimal photosystem II particles which are photochemically active contain three subunits: D1, D2 and cytochrome b559 (Cyt b559). The function of Cyt b559 remains unclear. We have successfully overexpressed the psbF gene, encoding the β subunit of Cyt b559, from a marine cyanobacterium Synechococcus sp. PCC 7002 as a fusion gene and obtained a redox-active form of Cyt b559. When the N-terminal GST protein of the fusion gene product was removed with thrombin, the PsbF protein was still redox-active, suggesting that the recombinant PsbF can form dimer in Escherichia coli. The absorption spectra of either the oxidized form or the reduced form of both GST fusion protein and the purified PsbF dimer and the difference spectra between the two forms are the same as that of the Cyt b559 isolated from the higher plants. Redox titration analysis of recombinant PsbF showed that the mid-point redox potential of the recombinant Cyt b559 was approximately 50 mV, which is close to the low potential of Cyt b559. The results are helpful to the understanding of localization and function of Cyt b559 on thylakoid membranes.
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